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1irz

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[[Image:1irz.gif|left|200px]]
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{{STRUCTURE_1irz| PDB=1irz | SCENE= }}
{{STRUCTURE_1irz| PDB=1irz | SCENE= }}
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'''Solution structure of ARR10-B belonging to the GARP family of plant Myb-related DNA binding motifs of the Arabidopsis response regulators'''
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===Solution structure of ARR10-B belonging to the GARP family of plant Myb-related DNA binding motifs of the Arabidopsis response regulators===
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==Overview==
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The B motif is a signature of type-B response regulators (ARRs) involved in His-to-Asp phosphorelay signal transduction systems in Arabidopsis. Homologous motifs occur widely in the GARP family of plant transcription factors. To gain general insight into the structure and function of B motifs (or GARP motifs), we characterized the B motif derived from a representative ARR, ARR10, which led to a number of intriguing findings. First, the B motif of ARR10 (named ARR10-B and extending from Thr-179 to Ser-242) possesses a nuclear localization signal, as indicated by the intracellular localization of a green fluorescent protein-ARR10-B fusion protein in onion epidermal cells. Second, the purified ARR10-B molecule binds specifically in vitro to DNA with the core sequence AGATT. This was demonstrated by several in vitro approaches, including PCR-assisted DNA binding site selection, gel retardation assays, and surface plasmon resonance analysis. Finally, the three-dimensional structure of ARR10-B in solution was determined by NMR spectroscopy, showing that it contains a helix-turn-helix structure. Furthermore, the mode of interaction between ARR10-B and the target DNA was assessed extensively by NMR spectroscopy. Together, these results lead us to propose that the mechanism of DNA recognition by ARR10-B is essentially the same as that of homeodomains. We conclude that the B motif is a multifunctional domain responsible for both nuclear localization and DNA binding and suggest that these insights could be applicable generally to the large GARP family of plant transcription factors.
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(as it appears on PubMed at http://www.pubmed.gov), where 12215502 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12215502}}
==About this Structure==
==About this Structure==
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1IRZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IRZ OCA].
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1IRZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IRZ OCA].
==Reference==
==Reference==
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[[Category: Yamazaki, T.]]
[[Category: Yamazaki, T.]]
[[Category: Helix-turn-helix]]
[[Category: Helix-turn-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:20:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:53:55 2008''

Revision as of 10:53, 1 July 2008

Image:1irz.png

Template:STRUCTURE 1irz

Solution structure of ARR10-B belonging to the GARP family of plant Myb-related DNA binding motifs of the Arabidopsis response regulators

Template:ABSTRACT PUBMED 12215502

About this Structure

1IRZ is a Single protein structure of sequence from Arabidopsis thaliana. Full experimental information is available from OCA.

Reference

Molecular structure of the GARP family of plant Myb-related DNA binding motifs of the Arabidopsis response regulators., Hosoda K, Imamura A, Katoh E, Hatta T, Tachiki M, Yamada H, Mizuno T, Yamazaki T, Plant Cell. 2002 Sep;14(9):2015-29. PMID:12215502

Page seeded by OCA on Tue Jul 1 13:53:55 2008

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