1iuq

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[[Image:1iuq.gif|left|200px]]
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{{Seed}}
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[[Image:1iuq.png|left|200px]]
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{{STRUCTURE_1iuq| PDB=1iuq | SCENE= }}
{{STRUCTURE_1iuq| PDB=1iuq | SCENE= }}
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'''The 1.55 A Crystal Structure of Glycerol-3-Phosphate Acyltransferase'''
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===The 1.55 A Crystal Structure of Glycerol-3-Phosphate Acyltransferase===
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==Overview==
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Stromal glycerol-3-phosphate acyltransferases (GPAT) are responsible for the selective incorporation of saturated and unsaturated fatty-acyl chains into chloroplast membranes, which is an important determinant of a plant's ability to tolerate chilling temperatures. The molecular mechanisms of plant chilling tolerance were elucidated by creating chimeric GPATs between squash (Cucurbita moscata, chilling-sensitive) and spinach (Spinacea oleracea, chilling-tolerant) and the results were interpreted using structural information on squash GPAT determined by X-ray crystallography at 1.55 A resolution. Enzymatic analysis of the chimeric GPATs showed that the chimeric GPATs containing the spinach region from residues 128 to 187 prefer the 18:1 unsaturated fatty acid rather than 16:0 saturated fatty acid. Structure analysis suggests that the size and character of the cavity that is formed from this region determines the specific recognition of acyl chains.
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(as it appears on PubMed at http://www.pubmed.gov), where 14684887 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14684887}}
==About this Structure==
==About this Structure==
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[[Category: Four helix bundle]]
[[Category: Four helix bundle]]
[[Category: Open twisted alpha/beta]]
[[Category: Open twisted alpha/beta]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:26:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:02:09 2008''

Revision as of 11:02, 1 July 2008

Image:1iuq.png

Template:STRUCTURE 1iuq

The 1.55 A Crystal Structure of Glycerol-3-Phosphate Acyltransferase

Template:ABSTRACT PUBMED 14684887

About this Structure

1IUQ is a Single protein structure of sequence from Cucurbita moschata. Full crystallographic information is available from OCA.

Reference

Substrate recognition and selectivity of plant glycerol-3-phosphate acyltransferases (GPATs) from Cucurbita moscata and Spinacea oleracea., Tamada T, Feese MD, Ferri SR, Kato Y, Yajima R, Toguri T, Kuroki R, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):13-21. Epub 2003, Dec 18. PMID:14684887

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