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1iy8

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[[Image:1iy8.jpg|left|200px]]
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{{STRUCTURE_1iy8| PDB=1iy8 | SCENE= }}
{{STRUCTURE_1iy8| PDB=1iy8 | SCENE= }}
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'''Crystal Structure of Levodione Reductase'''
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===Crystal Structure of Levodione Reductase===
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==Overview==
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The (6R)-2,2,6-trimethyl-1,4-cyclohexanedione (levodione) reductase (LVR) of the soil isolate bacterium Corynebacterium aquaticum M-13 is a NAD(H)-linked enzyme that catalyzes reversible oxidoreduction between (4R)-hydroxy-(6R)-2,2,6-trimethylcyclohexanone (actinol) and levodione. Here the crystal structure of a ternary complex of LVR with NADH and its inhibitor 2-methyl-2,4-pentanediol has been determined by molecular replacement and refined at 1.6-A resolution with a crystallographic R factor of 0.199. The overall structure is similar to those of other short-chain alcohol dehydrogenase/reductase enzymes. The positions of NADH and 2-methyl-2,4-pentanediol indicate the binding site of the substrate and identify residues that are likely to be important in the catalytic reaction. Modeling of the substrate binding in the active site suggests that the specificity of LVR is determined by electrostatic interactions between the negatively charged surface of Glu-103 of LVR and the positively charged surface on the re side of levodione. Mutant LVR enzymes in which Glu-103 is substituted with alanine (E103A), glutamine (E103Q), asparagines (E103N), or aspartic acid (E103D) show a 2-6-fold increase in Km values as compared with wild-type LVR and a much lower enantiomeric excess of the reaction products (60%) than the wild-type enzyme (95%). Together, these data indicate that Glu-103 has an important role in determining the stereospecificity of LVR.
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(as it appears on PubMed at http://www.pubmed.gov), where 12621044 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12621044}}
==About this Structure==
==About this Structure==
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[[Category: Yoshizumi, A.]]
[[Category: Yoshizumi, A.]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:12:40 2008''

Revision as of 11:12, 1 July 2008

Image:1iy8.png

Template:STRUCTURE 1iy8

Crystal Structure of Levodione Reductase

Template:ABSTRACT PUBMED 12621044

About this Structure

1IY8 is a Single protein structure of sequence from Leifsonia aquatica. Full crystallographic information is available from OCA.

Reference

The crystal structure and stereospecificity of levodione reductase from Corynebacterium aquaticum M-13., Sogabe S, Yoshizumi A, Fukami TA, Shiratori Y, Shimizu S, Takagi H, Nakamori S, Wada M, J Biol Chem. 2003 May 23;278(21):19387-95. Epub 2003 Mar 5. PMID:12621044

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