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1iyw

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{{STRUCTURE_1iyw| PDB=1iyw | SCENE= }}
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'''Preliminary Structure of Thermus thermophilus Ligand-Free Valyl-tRNA Synthetase'''
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===Preliminary Structure of Thermus thermophilus Ligand-Free Valyl-tRNA Synthetase===
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==Overview==
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The molecular interactions between valyl-tRNA synthetase (ValRS) and tRNA(Val), with the C34-A35-C36 anticodon, from Thermus thermophilus were studied by crystallographic analysis and structure-based mutagenesis. In the ValRS-bound structure of tRNA(Val), the successive A35-C36 residues (the major identity elements) of tRNA(Val) are base-stacked upon each other, and fit into a pocket on the alpha-helix bundle domain of ValRS. Hydrogen bonds are formed between ValRS and A35-C36 of tRNA(Val) in a base-specific manner. The C-terminal coiled-coil domain of ValRS interacts electrostatically with A20 and hydrophobically with the G19*C56 tertiary base pair. The loss of these interactions by the deletion of the coiled-coil domain of ValRS increased the K(M) value for tRNA(Val) 28-fold and decreased the k(cat) value 19-fold in the aminoacylation. The tRNA(Val) K(M) and k(cat) values were increased 21-fold and decreased 32-fold, respectively, by the disruption of the G18*U55 and G19*C56 tertiary base pairs, which associate the D- and T-loops for the formation of the L-shaped tRNA structure. Therefore, the coiled-coil domain of ValRS is likely to stabilize the L-shaped tRNA structure during the aminoacylation reaction.
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{{ABSTRACT_PUBMED_12554880}}
==About this Structure==
==About this Structure==
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[[Category: Rsgi]]
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
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Revision as of 11:14, 1 July 2008

Image:1iyw.png

Template:STRUCTURE 1iyw

Preliminary Structure of Thermus thermophilus Ligand-Free Valyl-tRNA Synthetase

Template:ABSTRACT PUBMED 12554880

About this Structure

1IYW is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Mechanism of molecular interactions for tRNA(Val) recognition by valyl-tRNA synthetase., Fukai S, Nureki O, Sekine S, Shimada A, Vassylyev DG, Yokoyama S, RNA. 2003 Jan;9(1):100-11. PMID:12554880

Page seeded by OCA on Tue Jul 1 14:14:36 2008

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