1j0q

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1j0q.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1j0q.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1j0q| PDB=1j0q | SCENE= }}
{{STRUCTURE_1j0q| PDB=1j0q | SCENE= }}
-
'''Solution Structure of Oxidized Bovine Microsomal Cytochrome b5 mutant V61H'''
+
===Solution Structure of Oxidized Bovine Microsomal Cytochrome b5 mutant V61H===
-
==Overview==
+
<!--
-
Using 1488 NOE constraints, 19 stereo-specific assignments, 13 pairs of H-bond constraints, and 140 pseudo-contact shift constraints, a family of 35 structures of bovine microsomal cytochrome b(5) mutant V61H has been obtained through the program PSEUDYANA. The family has been further refined by restrained energy minimization to give a family of final structures. The RMSD values of final structures with respect to the average structure are 0.45+/-0.11 and 0.96+/-0.10A for backbone and heavy atoms, respectively. The final Deltachi(ax) and Deltachi(rh) values are 2.34 x 10(-32) and -0.67 x 10(-32)m(3), respectively. The comparisons between the solution structures of mutant V61H and WT cytochrome b(5), and X-ray structure of the mutant V61H show that the global folding of the molecule in solution is unchanged and the side-chain of His61 deviates from the heme pocket and extends into the solvent like in its crystal structure. However, the helices around the heme pocket undergo outward global displacement while their local conformations are well maintained. Meanwhile, the heme ring shows a little off the heme pocket, which accounts for the lower stability of the mutant. Additionally, the axial ligand rings counterclockwise rotate around His39 N-Fe axis due to the mutation, which is confirmed by variation of the hyperfine shifts of the heme protons of V61H compared to those of WT cytochrome b(5).
+
The line below this paragraph, {{ABSTRACT_PUBMED_12893266}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12893266 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12893266}}
==About this Structure==
==About this Structure==
-
1J0Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0Q OCA].
+
1J0Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0Q OCA].
==Reference==
==Reference==
Line 31: Line 35:
[[Category: Beta]]
[[Category: Beta]]
[[Category: Helix]]
[[Category: Helix]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:39:43 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:19:21 2008''

Revision as of 11:19, 1 July 2008

Image:1j0q.png

Template:STRUCTURE 1j0q

Solution Structure of Oxidized Bovine Microsomal Cytochrome b5 mutant V61H

Template:ABSTRACT PUBMED 12893266

About this Structure

1J0Q is a Single protein structure of sequence from Bos taurus. Full experimental information is available from OCA.

Reference

The solution structure of the oxidized bovine microsomal cytochrome b(5) mutant V61H., Cao C, Zhang Q, Xue LL, Ma J, Wang YH, Wu H, Huang ZX, Biochem Biophys Res Commun. 2003 Aug 1;307(3):600-9. PMID:12893266

Page seeded by OCA on Tue Jul 1 14:19:21 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1j0q"
Personal tools