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1j3i

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[[Image:1j3i.gif|left|200px]]
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{{STRUCTURE_1j3i| PDB=1j3i | SCENE= }}
{{STRUCTURE_1j3i| PDB=1j3i | SCENE= }}
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'''Wild-type Plasmodium falciparum dihydrofolate reductase-thymidylate synthase (PfDHFR-TS) complexed with WR99210, NADPH, and dUMP'''
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===Wild-type Plasmodium falciparum dihydrofolate reductase-thymidylate synthase (PfDHFR-TS) complexed with WR99210, NADPH, and dUMP===
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==Overview==
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Plasmodium falciparum dihydrofolate reductase-thymidylate synthase (PfDHFR-TS) is an important target of antimalarial drugs. The efficacy of this class of DHFR-inhibitor drugs is now compromised because of mutations that prevent drug binding yet retain enzyme activity. The crystal structures of PfDHFR-TS from the wild type (TM4/8.2) and the quadruple drug-resistant mutant (V1/S) strains, in complex with a potent inhibitor WR99210, as well as the resistant double mutant (K1 CB1) with the antimalarial pyrimethamine, reveal features for overcoming resistance. In contrast to pyrimethamine, the flexible side chain of WR99210 can adopt a conformation that fits well in the active site, thereby contributing to binding. The single-chain bifunctional PfDHFR-TS has a helical insert between the DHFR and TS domains that is involved in dimerization and domain organization. Moreover, positively charged grooves on the surface of the dimer suggest a function in channeling of substrate from TS to DHFR active sites. These features provide possible approaches for the design of new drugs to overcome antifolate resistance.
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(as it appears on PubMed at http://www.pubmed.gov), where 12704428 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12704428}}
==About this Structure==
==About this Structure==
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[[Category: Yuvaniyama, J.]]
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[[Category: Bifunctional]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:45:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:26:51 2008''

Revision as of 11:26, 1 July 2008

Image:1j3i.png

Template:STRUCTURE 1j3i

Wild-type Plasmodium falciparum dihydrofolate reductase-thymidylate synthase (PfDHFR-TS) complexed with WR99210, NADPH, and dUMP

Template:ABSTRACT PUBMED 12704428

About this Structure

1J3I is a Protein complex structure of sequences from Plasmodium falciparum. Full crystallographic information is available from OCA.

Reference

Insights into antifolate resistance from malarial DHFR-TS structures., Yuvaniyama J, Chitnumsub P, Kamchonwongpaisan S, Vanichtanankul J, Sirawaraporn W, Taylor P, Walkinshaw MD, Yuthavong Y, Nat Struct Biol. 2003 May;10(5):357-65. PMID:12704428

Page seeded by OCA on Tue Jul 1 14:26:51 2008

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