1j5k

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{{STRUCTURE_1j5k| PDB=1j5k | SCENE= }}
{{STRUCTURE_1j5k| PDB=1j5k | SCENE= }}
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'''COMPLEX OF THE KH3 DOMAIN OF HNRNP K WITH A SINGLE_STRANDED 10MER DNA OLIGONUCLEOTIDE'''
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===COMPLEX OF THE KH3 DOMAIN OF HNRNP K WITH A SINGLE_STRANDED 10MER DNA OLIGONUCLEOTIDE===
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==Overview==
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To elucidate the basis of sequence-specific single-stranded (ss) DNA recognition by K homology (KH) domains, we have solved the solution structure of a complex between the KH3 domain of the transcriptional regulator heterogeneous nuclear ribonucleoprotein K (hnRNP K) and a 10mer ssDNA. We show that hnRNP K KH3 specifically recognizes a tetrad of sequence 5'd-TCCC. The complex is stabilized by a dense network of methyl-oxygen hydrogen bonds involving the methyl groups of three isoleucine residues and the O2 and N3 atoms of the two central cytosine bases. Comparison with the recently solved structure of a specific protein-ssDNA complex involving the KH3 and KH4 domains of the far upstream element (FUSE) binding protein FBP suggests that the amino acid located five residues N-terminal of the invariant GXXG motif, which is characteristic of all KH domains, plays a crucial role in discrimination of the first two bases of the tetrad.
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(as it appears on PubMed at http://www.pubmed.gov), where 12093748 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12093748}}
==About this Structure==
==About this Structure==
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1J5K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5K OCA].
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1J5K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5K OCA].
==Reference==
==Reference==
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[[Category: Single-stranded dna binding protein]]
[[Category: Single-stranded dna binding protein]]
[[Category: Transcription factor]]
[[Category: Transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:49:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:32:06 2008''

Revision as of 11:32, 1 July 2008

Image:1j5k.png

Template:STRUCTURE 1j5k

COMPLEX OF THE KH3 DOMAIN OF HNRNP K WITH A SINGLE_STRANDED 10MER DNA OLIGONUCLEOTIDE

Template:ABSTRACT PUBMED 12093748

About this Structure

1J5K is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Molecular basis of sequence-specific single-stranded DNA recognition by KH domains: solution structure of a complex between hnRNP K KH3 and single-stranded DNA., Braddock DT, Baber JL, Levens D, Clore GM, EMBO J. 2002 Jul 1;21(13):3476-85. PMID:12093748

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