1j7m

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{{STRUCTURE_1j7m| PDB=1j7m | SCENE= }}
{{STRUCTURE_1j7m| PDB=1j7m | SCENE= }}
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'''The Third Fibronectin Type II Module from Human Matrix Metalloproteinase 2'''
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===The Third Fibronectin Type II Module from Human Matrix Metalloproteinase 2===
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==Overview==
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Human matrix metalloproteinase-2 (MMP-2) contains an array of three fibronectin type II (FII) modules postulated to interact with gelatin (denatured collagen). Here, we verify that the NMR solution structure of the third FII repeat (COL-3) is similar to that of the second FII repeat (COL-2); characterize its ligand-binding properties; and derive dynamics properties and relative orientation in solution for the two domains of the COL-23 fragment, a construct comprising COL-2 and COL-3 in tandem, with each domain possessing a putative collagen-binding site. Interaction of the synthetic gelatin-like octadecapeptide (Pro-Pro-Gly)(6) (PPG6) with COL-3 is weaker than with COL-2. We found that a synthetic peptide comprising segment 33-42 (peptide 33-42) from the MMP-2 prodomain interacts with COL-3 and, albeit with lower affinity, with COL-2 in a way that mimics PPG6 binding. COL-3 strongly prefers peptide 33-42 over PPG6, which suggests that intramolecular interactions with the prodomain could modulate binding of pro-MMP-2 to its gelatin substrate. In COL-23, the two modules retain their structural individuality and tumble independently. Overall, the NMR data indicate that the relative orientation of the modules in COL-23 is not fixed in solution, that the modules do not interact with one another, and that COL-23 is rather flexible. The binding sites face opposite each other, and their responses to, and normalized affinities for, the longer ligand PPG12 are virtually identical to those of the individual domains for PPG6, thus precluding co- operativity, although they may interact simultaneously with multiple sites of the extracellular matrix.
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(as it appears on PubMed at http://www.pubmed.gov), where 11320090 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11320090}}
==About this Structure==
==About this Structure==
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1J7M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J7M OCA].
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1J7M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J7M OCA].
==Reference==
==Reference==
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[[Category: Alpha helix]]
[[Category: Alpha helix]]
[[Category: Beta sheet]]
[[Category: Beta sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:53:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:35:45 2008''

Revision as of 11:35, 1 July 2008

Image:1j7m.png

Template:STRUCTURE 1j7m

The Third Fibronectin Type II Module from Human Matrix Metalloproteinase 2

Template:ABSTRACT PUBMED 11320090

About this Structure

1J7M is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Gelatin-binding region of human matrix metalloproteinase-2: solution structure, dynamics, and function of the COL-23 two-domain construct., Briknarova K, Gehrmann M, Banyai L, Tordai H, Patthy L, Llinas M, J Biol Chem. 2001 Jul 20;276(29):27613-21. Epub 2001 Apr 24. PMID:11320090

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