1gxp
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1gxp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gxp, resolution 2.5Å" /> '''PHOB EFFECTOR DOMAIN ...)
Next diff →
Revision as of 14:12, 20 November 2007
|
PHOB EFFECTOR DOMAIN IN COMPLEX WITH PHO BOX DNA.
Overview
PhoB is a signal transduction response regulator that activates nearly 40, genes in phosphate depletion conditions in E. coli and closely related, bacteria. The structure of the PhoB effector domain in complex with its, target DNA sequence, or pho box, reveals a novel tandem arrangement in, which several monomers bind head to tail to successive 11-base pair, direct-repeat sequences, coating one face of a smoothly bent double helix., The protein has a winged helix fold in which the DNA recognition elements, comprise helix alpha 3, penetrating the major groove, and a beta hairpin, wing interacting with a compressed minor groove via Arg219, tightly, sandwiched between the DNA sugar backbones. The transactivation loops, protrude laterally in an appropriate orientation to interact with the RNA, polymerase sigma(70) subunit, which triggers transcription initiation.
About this Structure
1GXP is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Tandem DNA recognition by PhoB, a two-component signal transduction transcriptional activator., Blanco AG, Sola M, Gomis-Ruth FX, Coll M, Structure. 2002 May;10(5):701-13. PMID:12015152
Page seeded by OCA on Tue Nov 20 16:20:11 2007
