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| - | [[Image:1jb5.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1jb5| PDB=1jb5 | SCENE= }} | | {{STRUCTURE_1jb5| PDB=1jb5 | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF NTF2 M118E MUTANT'''
| + | ===CRYSTAL STRUCTURE OF NTF2 M118E MUTANT=== |
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| - | ==Overview==
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| - | Nuclear transport factor 2 (NTF2) mediates nuclear import of RanGDP, a central component of many nuclear trafficking pathways. NTF2 is a homodimer and each chain has independent binding sites for RanGDP and nuclear pore proteins (nucleoporins) that contain FxFG sequence repeats. We show here that the monomer-dimer dissociation constant for NTF2 obtained by sedimentation equilibrium ultracentrifugation is in the micromolar range, indicating that a substantial proportion of cellular NTF2 may be monomeric. To investigate the functional significance of NTF2 dimerization, we engineered a series of point mutations at the dimerization interface and one of these (M118E) remained monomeric below concentrations of 150 microM. CD spectra and X-ray crystallography showed that M118E-NTF2 preserved the wild-type NTF2 fold, although its thermal stability was 20 deg. C lower than that of the wild-type. M118E-NTF2 bound both RanGDP and FxFG nucleoporins less strongly, suggesting that dissociation of the NTF2 dimer could facilitate RanGDP release and thus nucleotide exchange after it had been transported into the nucleus. Moreover, colloidal gold coated with M118E-NTF2 showed reduced binding to Xenopus oocyte nuclear pores. Overall, our results indicate that dimer formation is important for NTF2 function and give insight into the formation of heterodimers by mRNA export factors such as TAP1 and NXT1 that contain NTF2-homology domains.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11846560}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11846560 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11846560}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Ntf2]] | | [[Category: Ntf2]] |
| | [[Category: Transport]] | | [[Category: Transport]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:00:18 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 19:56:59 2008'' |
Revision as of 16:57, 1 July 2008
Template:STRUCTURE 1jb5
CRYSTAL STRUCTURE OF NTF2 M118E MUTANT
Template:ABSTRACT PUBMED 11846560
About this Structure
1JB5 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
NTF2 monomer-dimer equilibrium., Chaillan-Huntington C, Butler PJ, Huntington JA, Akin D, Feldherr C, Stewart M, J Mol Biol. 2001 Nov 30;314(3):465-77. PMID:11846560
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