1jb9

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{{STRUCTURE_1jb9| PDB=1jb9 | SCENE= }}
{{STRUCTURE_1jb9| PDB=1jb9 | SCENE= }}
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'''Crystal Structure of The Ferredoxin:NADP+ Reductase From Maize Root AT 1.7 Angstroms'''
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===Crystal Structure of The Ferredoxin:NADP+ Reductase From Maize Root AT 1.7 Angstroms===
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==Overview==
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Distinct forms of ferredoxin-NADP(+) reductase are expressed in photosynthetic and nonphotosynthetic plant tissues. Both enzymes catalyze electron transfer between NADP(H) and ferredoxin; whereas in leaves the enzyme transfers reducing equivalents from photoreduced ferredoxin to NADP(+) in photosynthesis, in roots it has the opposite physiological role, reducing ferredoxin at the expense of NADPH mainly for use in nitrate assimilation. Here, structural and kinetic properties of a nonphotosynthetic isoform were analyzed to define characteristics that may be related to tissue-specific function. Compared with spinach leaf ferredoxin-NADP(+) reductase, the recombinant corn root isoform showed a slightly altered absorption spectrum, a higher pI, a &gt;30-fold higher affinity for NADP(+), greater susceptibility to limited proteolysis, and an approximately 20 mV more positive redox potential. The 1.7 A resolution crystal structure is very similar to the structures of ferredoxin-NADP(+) reductases from photosynthetic tissues. Four distinct structural features of this root ferredoxin-NADP(+) reductases are an alternate conformation of the bound FAD molecule, an alternate path for the amino-terminal extension, a disulfide bond in the FAD-binding domain, and changes in the surface that binds ferredoxin.
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(as it appears on PubMed at http://www.pubmed.gov), where 11724563 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11724563}}
==About this Structure==
==About this Structure==
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[[Category: Spinola, M.]]
[[Category: Spinola, M.]]
[[Category: Electron transport]]
[[Category: Electron transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:00:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 19:57:38 2008''

Revision as of 16:57, 1 July 2008

Image:1jb9.png

Template:STRUCTURE 1jb9

Crystal Structure of The Ferredoxin:NADP+ Reductase From Maize Root AT 1.7 Angstroms

Template:ABSTRACT PUBMED 11724563

About this Structure

1JB9 is a Single protein structure of sequence from Zea mays. Full crystallographic information is available from OCA.

Reference

Biochemical and crystallographic characterization of ferredoxin-NADP(+) reductase from nonphotosynthetic tissues., Aliverti A, Faber R, Finnerty CM, Ferioli C, Pandini V, Negri A, Karplus PA, Zanetti G, Biochemistry. 2001 Dec 4;40(48):14501-8. PMID:11724563

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