1jcm

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[[Image:1jcm.gif|left|200px]]
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{{STRUCTURE_1jcm| PDB=1jcm | SCENE= }}
{{STRUCTURE_1jcm| PDB=1jcm | SCENE= }}
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'''TRPC STABILITY MUTANT CONTAINING AN ENGINEERED DISULPHIDE BRIDGE AND IN COMPLEX WITH A CDRP-RELATED SUBSTRATE'''
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===TRPC STABILITY MUTANT CONTAINING AN ENGINEERED DISULPHIDE BRIDGE AND IN COMPLEX WITH A CDRP-RELATED SUBSTRATE===
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==Overview==
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The aim of this study was to increase the stability of the thermolabile (betaalpha)8-barrel enzyme indoleglycerol phosphate synthase from Escherichia coli by the introduction of disulfide bridges. For the design of such variants, we selected two out of 12 candidates, in which newly introduced cysteines potentially form optimal disulfide bonds. These variants avoid short-range connections, substitutions near catalytic residues, and crosslinks between the new and the three parental cysteines. The variant linking residues 3 and 189 fastens the N-terminus to the (betaalpha)8-barrel. The rate of thermal inactivation at 50 degrees C of this variant with a closed disulfide bridge is 65-fold slower than that of the reference dithiol form, but only 13-fold slower than that of the parental protein. The near-ultraviolet CD spectrum, the reactivity of parental buried cysteines with Ellman's reagent as well as the decreased turnover number indicate that the protein structure is rigidified. To confirm these data, we have solved the X-ray structure to 2.1-A resolution. The second variant was designed to crosslink the terminal modules betaalpha1 and betaalpha8. However, not even the dithiol form acquired the native fold, possibly because one of the targeted residues is solvent-inaccessible in the parental protein.
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The line below this paragraph, {{ABSTRACT_PUBMED_11856350}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11856350 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11856350}}
==About this Structure==
==About this Structure==
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[[Category: Disulphide bridge]]
[[Category: Disulphide bridge]]
[[Category: Stability mutant]]
[[Category: Stability mutant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:03:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:01:46 2008''

Revision as of 17:01, 1 July 2008

Image:1jcm.png

Template:STRUCTURE 1jcm

TRPC STABILITY MUTANT CONTAINING AN ENGINEERED DISULPHIDE BRIDGE AND IN COMPLEX WITH A CDRP-RELATED SUBSTRATE

Template:ABSTRACT PUBMED 11856350

About this Structure

1JCM is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Stabilization of a (betaalpha)8-barrel protein by an engineered disulfide bridge., Ivens A, Mayans O, Szadkowski H, Jurgens C, Wilmanns M, Kirschner K, Eur J Biochem. 2002 Feb;269(4):1145-53. PMID:11856350

Page seeded by OCA on Tue Jul 1 20:01:46 2008

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