1jef

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{{STRUCTURE_1jef| PDB=1jef | SCENE= }}
{{STRUCTURE_1jef| PDB=1jef | SCENE= }}
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'''TURKEY LYSOZYME COMPLEX WITH (GLCNAC)3'''
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===TURKEY LYSOZYME COMPLEX WITH (GLCNAC)3===
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==Overview==
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The turkey-egg lysozyme (TEL) complex with tri-N-acetylchitotriose [(GlcNac)3] was co-crystallized from 1.5% TEL and 2 mM (GlcNac)3 at pH 4.2. The crystal structure was determined by molecular replacement and refined to an R value of 0.182 using 10-1.77 A data. The (GlcNac)3 molecule occupies the subsites A, B and C. At the subsites B and C, the sugar residues are bound in a similar manner to that found in the hen-egg lysozyme (HEL) complex. In contrast, the GlcNac residue at the subsite A is exposed to bulk solvent and has no contact with the protein molecule because the active residue Asp101 in HEL is replaced by Gly in TEL. A sulfate ion is bound in the vicinity of subsite B and forms hydrogen bonds with the sugar residue and the guanidino group of Arg61, assisting the binding of the sugar residue to subsite B. The active-site cleft of TEL is narrower than that of native TEL, thus attaining the best fit of the (GlcNac)3 molecule. The lack of binding ability of subsite A is discussed in relation to the catalytic properties of TEL. The result suggests that the cleavage pattern of oligosaccharide substrates in the catalytic reaction is regulated by the protein-sugar interaction at subsite A.
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(as it appears on PubMed at http://www.pubmed.gov), where 15299852 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15299852}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Inhibitor complex]]
[[Category: Inhibitor complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:07:00 2008''

Revision as of 17:07, 1 July 2008

Image:1jef.png

Template:STRUCTURE 1jef

TURKEY LYSOZYME COMPLEX WITH (GLCNAC)3

Template:ABSTRACT PUBMED 15299852

About this Structure

1JEF is a Single protein structure of sequence from Meleagris gallopavo. Full crystallographic information is available from OCA.

Reference

X-ray structure of turkey-egg lysozyme complex with tri-N-acetylchitotriose. Lack of binding ability at subsite A., Harata K, Muraki M, Acta Crystallogr D Biol Crystallogr. 1997 Nov 1;53(Pt 6):650-7. PMID:15299852

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