1jf5

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{{STRUCTURE_1jf5| PDB=1jf5 | SCENE= }}
{{STRUCTURE_1jf5| PDB=1jf5 | SCENE= }}
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'''CRYSTAL STRUCTURE OF THERMOACTINOMYCES VULGARIS R-47 ALPHA-AMYLASE 2 MUTANT F286A'''
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===CRYSTAL STRUCTURE OF THERMOACTINOMYCES VULGARIS R-47 ALPHA-AMYLASE 2 MUTANT F286A===
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==Overview==
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Phe286 located in the center of the active site of alpha-amylase 2 from Thermoactinomyces vulgaris R-47 (TVAII) plays an important role in the substrate recognition for cyclomaltooligosaccharides (cyclodextrins). The X-ray structures of mutant TVAIIs with the replacement of Phe286 by Ala (F286A) and Tyr (F286Y) were determined at 3.2 A resolution. Their structures have no significant differences from that of the wild-type enzyme. The kinetic analyses of Phe286-replaced variants showed that the variants with non-aromatic residues, Ala (F286A) and Leu (F286L), have lower enzymatic activities than those with aromatic residues, Tyr (F286Y) and Trp (F286W), and the replacement of Phe286 affects enzymatic activities for CDs more than those for starch.
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(as it appears on PubMed at http://www.pubmed.gov), where 11527532 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11527532}}
==About this Structure==
==About this Structure==
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[[Category: Tonozuka, T.]]
[[Category: Tonozuka, T.]]
[[Category: Beta/alpha barrel]]
[[Category: Beta/alpha barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:09:07 2008''

Revision as of 17:09, 1 July 2008

Image:1jf5.png

Template:STRUCTURE 1jf5

CRYSTAL STRUCTURE OF THERMOACTINOMYCES VULGARIS R-47 ALPHA-AMYLASE 2 MUTANT F286A

Template:ABSTRACT PUBMED 11527532

About this Structure

1JF5 is a Single protein structure of sequence from Thermoactinomyces vulgaris. Full crystallographic information is available from OCA.

Reference

Role of Phe286 in the recognition mechanism of cyclomaltooligosaccharides (cyclodextrins) by Thermoactinomyces vulgaris R-47 alpha-amylase 2 (TVAII). X-ray structures of the mutant TVAIIs, F286A and F286Y, and kinetic analyses of the Phe286-replaced mutant TVAIIs., Ohtaki A, Kondo S, Shimura Y, Tonozuka T, Sakano Y, Kamitori S, Carbohydr Res. 2001 Sep 7;334(4):309-13. PMID:11527532

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