1jfh

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{{STRUCTURE_1jfh| PDB=1jfh | SCENE= }}
{{STRUCTURE_1jfh| PDB=1jfh | SCENE= }}
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'''STRUCTURE OF A PANCREATIC ALPHA-AMYLASE BOUND TO A SUBSTRATE ANALOGUE AT 2.03 ANGSTROM RESOLUTION'''
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===STRUCTURE OF A PANCREATIC ALPHA-AMYLASE BOUND TO A SUBSTRATE ANALOGUE AT 2.03 ANGSTROM RESOLUTION===
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==Overview==
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The structure of pig pancreatic alpha-amylase in complex with carbohydrate inhibitor and proteinaceous inhibitors is known but the successive events occurring at the catalytic center still remain to be elucidated. The X-ray structure analysis of a crystal of pig pancreatic alpha-amylase (PPA, EC 3.2.1.1.) soaked with an enzyme-resistant substrate analogue, methyl 4,4'-dithio-alpha-maltotrioside, showed electron density corresponding to the binding of substrate analogue molecules at the active site and at the "second binding site." The electron density observed at the active site was interpreted in terms of overlapping networks of oligosaccharides, which show binding of substrate analogue molecules at subsites prior to and subsequent to the cleavage site. A weaker patch of density observed at subsite -1 (using a nomenclature where the site of hydrolysis is taken to be between subsites -1 and +1) was modeled with water molecules. Conformational changes take place upon substrate analogue binding and the "flexible loop" that constitutes the surface edge of the active site is observed in a specific conformation. This confirms that this loop plays an important role in the recognition and binding of the ligand. The crystal structure was refined at 2.03 A resolution, to an R-factor of 16.0 (Rfree, 18.5).
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(as it appears on PubMed at http://www.pubmed.gov), where 9385631 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9385631}}
==About this Structure==
==About this Structure==
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[[Category: Methyl 4,4'-dithio-alpha-maltotrioside]]
[[Category: Methyl 4,4'-dithio-alpha-maltotrioside]]
[[Category: O-glycosyl]]
[[Category: O-glycosyl]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:09:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:10:08 2008''

Revision as of 17:10, 1 July 2008

Image:1jfh.png

Template:STRUCTURE 1jfh

STRUCTURE OF A PANCREATIC ALPHA-AMYLASE BOUND TO A SUBSTRATE ANALOGUE AT 2.03 ANGSTROM RESOLUTION

Template:ABSTRACT PUBMED 9385631

About this Structure

1JFH is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Structure of a pancreatic alpha-amylase bound to a substrate analogue at 2.03 A resolution., Qian M, Spinelli S, Driguez H, Payan F, Protein Sci. 1997 Nov;6(11):2285-96. PMID:9385631

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