1jg6

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{{Seed}}
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[[Image:1jg6.png|left|200px]]
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{{STRUCTURE_1jg6| PDB=1jg6 | SCENE= }}
{{STRUCTURE_1jg6| PDB=1jg6 | SCENE= }}
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'''T4 phage BGT in complex with UDP'''
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===T4 phage BGT in complex with UDP===
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==Overview==
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beta-Glucosyltransferase (BGT) is a DNA-modifying enzyme encoded by bacteriophage T4 that transfers glucose from uridine diphosphoglucose to 5-hydroxymethyl cytosine bases of phage T4 DNA. We report six X-ray structures of the substrate-free and the UDP-bound enzyme. Four also contain metal ions which activate the enzyme, including Mg(2+) in forms 1 and 2 and Mn(2+) or Ca(2+). The substrate-free BGT structure differs by a domain movement from one previously determined in another space group. Further domain movements are seen in the complex with UDP and the four UDP-metal complexes. Mg(2+), Mn(2+) and Ca(2+) bind near the beta-phosphate of the nucleotide, but they occupy slightly different positions and have different ligands depending on the metal and the crystal form. Whilst the metal site observed in these complexes with the product UDP is not compatible with a role in activating glucose transfer, it approximates the position of the positive charge in the oxocarbonium ion thought to form on the glucose moiety of the substrate during catalysis.
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The line below this paragraph, {{ABSTRACT_PUBMED_11493010}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11493010 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11493010}}
==About this Structure==
==About this Structure==
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[[Category: Ruger, W.]]
[[Category: Ruger, W.]]
[[Category: Glycosyltransferase]]
[[Category: Glycosyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:11:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:11:54 2008''

Revision as of 17:11, 1 July 2008

Image:1jg6.png

Template:STRUCTURE 1jg6

T4 phage BGT in complex with UDP

Template:ABSTRACT PUBMED 11493010

About this Structure

1JG6 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

High resolution crystal structures of T4 phage beta-glucosyltransferase: induced fit and effect of substrate and metal binding., Morera S, Lariviere L, Kurzeck J, Aschke-Sonnenborn U, Freemont PS, Janin J, Ruger W, J Mol Biol. 2001 Aug 17;311(3):569-77. PMID:11493010

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