1jgn

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[[Image:1jgn.jpg|left|200px]]
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{{STRUCTURE_1jgn| PDB=1jgn | SCENE= }}
{{STRUCTURE_1jgn| PDB=1jgn | SCENE= }}
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'''Solution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip2'''
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===Solution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip2===
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==Overview==
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The C-terminal domain of poly(A)-binding protein (PABC) is a peptide-binding domain found in poly(A)-binding proteins (PABPs) and a HECT (homologous to E6-AP C-terminus) family E3 ubiquitin ligase. In protein synthesis, the PABC domain of PABP functions to recruit several translation factors possessing the PABP-interacting motif 2 (PAM2) to the mRNA poly(A) tail. We have determined the solution structure of the human PABC domain in complex with two peptides from PABP-interacting protein-1 (Paip1) and Paip2. The structures show a novel mode of peptide recognition, in which the peptide binds as a pair of beta-turns with extensive hydrophobic, electrostatic and aromatic stacking interactions. Mutagenesis of PABC and peptide residues was used to identify key protein-peptide interactions and quantified by isothermal calorimetry, surface plasmon resonance and GST pull-down assays. The results provide insight into the specificity of PABC in mediating PABP-protein interactions.
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The line below this paragraph, {{ABSTRACT_PUBMED_14685257}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 14685257 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14685257}}
==About this Structure==
==About this Structure==
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1JGN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JGN OCA].
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1JGN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JGN OCA].
==Reference==
==Reference==
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[[Category: All-helical domain]]
[[Category: All-helical domain]]
[[Category: Protein-peptide complex]]
[[Category: Protein-peptide complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:12:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:12:56 2008''

Revision as of 17:12, 1 July 2008

Image:1jgn.png

Template:STRUCTURE 1jgn

Solution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip2

Template:ABSTRACT PUBMED 14685257

About this Structure

1JGN is a Protein complex structure of sequences from Homo sapiens. Full experimental information is available from OCA.

Reference

Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase., Kozlov G, De Crescenzo G, Lim NS, Siddiqui N, Fantus D, Kahvejian A, Trempe JF, Elias D, Ekiel I, Sonenberg N, O'Connor-McCourt M, Gehring K, EMBO J. 2004 Jan 28;23(2):272-81. Epub 2003 Dec 18. PMID:14685257

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