1jgw

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[[Image:1jgw.gif|left|200px]]
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{{Seed}}
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[[Image:1jgw.png|left|200px]]
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{{STRUCTURE_1jgw| PDB=1jgw | SCENE= }}
{{STRUCTURE_1jgw| PDB=1jgw | SCENE= }}
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'''Photosynthetic Reaction Center Mutant With Thr M 21 Replaced With Leu'''
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===Photosynthetic Reaction Center Mutant With Thr M 21 Replaced With Leu===
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==Overview==
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The role of contact interactions in the crystallization of membrane proteins was assessed by mutation of amino-acid residues on the surface of the reaction center from Rhodobacter sphaeroides. Five single-site mutants were constructed, with changes in contact regions found in the trigonal and tetragonal forms but not the orthorhombic form. Crystallization trials for the tetragonal form yielded either no crystals or crystals with an altered morphology, whereas crystals grew in the other two forms, indicating that these interactions are essential for the stability of the tetragonal crystals. Changes in the structures determined by X-ray diffraction of trigonal crystals for each mutant were related to the quality of the diffraction. Significant differences in the resolution limit of the crystals were associated with the loss of specific interactions between neighboring proteins. The results suggest that the contact regions are crucial for obtaining highly ordered crystals of membrane proteins.
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The line below this paragraph, {{ABSTRACT_PUBMED_11526320}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11526320 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11526320}}
==About this Structure==
==About this Structure==
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[[Category: Williams, J C.]]
[[Category: Williams, J C.]]
[[Category: Alpha helix]]
[[Category: Alpha helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:12:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:13:34 2008''

Revision as of 17:13, 1 July 2008

Image:1jgw.png

Template:STRUCTURE 1jgw

Photosynthetic Reaction Center Mutant With Thr M 21 Replaced With Leu

Template:ABSTRACT PUBMED 11526320

About this Structure

1JGW is a Protein complex structure of sequences from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.

Reference

Individual interactions influence the crystalline order for membrane proteins., Camara-Artigas A, Magee CL, Williams JC, Allen JP, Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1281-6. Epub 2001, Aug 23. PMID:11526320

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