This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1jh4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1jh4.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1jh4.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1jh4| PDB=1jh4 | SCENE= }}
{{STRUCTURE_1jh4| PDB=1jh4 | SCENE= }}
-
'''Solution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip1'''
+
===Solution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip1===
-
==Overview==
+
<!--
-
The C-terminal domain of poly(A)-binding protein (PABC) is a peptide-binding domain found in poly(A)-binding proteins (PABPs) and a HECT (homologous to E6-AP C-terminus) family E3 ubiquitin ligase. In protein synthesis, the PABC domain of PABP functions to recruit several translation factors possessing the PABP-interacting motif 2 (PAM2) to the mRNA poly(A) tail. We have determined the solution structure of the human PABC domain in complex with two peptides from PABP-interacting protein-1 (Paip1) and Paip2. The structures show a novel mode of peptide recognition, in which the peptide binds as a pair of beta-turns with extensive hydrophobic, electrostatic and aromatic stacking interactions. Mutagenesis of PABC and peptide residues was used to identify key protein-peptide interactions and quantified by isothermal calorimetry, surface plasmon resonance and GST pull-down assays. The results provide insight into the specificity of PABC in mediating PABP-protein interactions.
+
The line below this paragraph, {{ABSTRACT_PUBMED_14685257}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 14685257 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_14685257}}
==About this Structure==
==About this Structure==
-
1JH4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JH4 OCA].
+
1JH4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JH4 OCA].
==Reference==
==Reference==
Line 29: Line 33:
[[Category: All-helical domain]]
[[Category: All-helical domain]]
[[Category: Protein-peptide complex]]
[[Category: Protein-peptide complex]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:12:51 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:14:03 2008''

Revision as of 17:14, 1 July 2008

Image:1jh4.png

Template:STRUCTURE 1jh4

Solution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip1

Template:ABSTRACT PUBMED 14685257

About this Structure

1JH4 is a Protein complex structure of sequences from Homo sapiens. Full experimental information is available from OCA.

Reference

Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase., Kozlov G, De Crescenzo G, Lim NS, Siddiqui N, Fantus D, Kahvejian A, Trempe JF, Elias D, Ekiel I, Sonenberg N, O'Connor-McCourt M, Gehring K, EMBO J. 2004 Jan 28;23(2):272-81. Epub 2003 Dec 18. PMID:14685257

Page seeded by OCA on Tue Jul 1 20:14:03 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jh4"
Personal tools