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| | {{STRUCTURE_1ji6| PDB=1ji6 | SCENE= }} | | {{STRUCTURE_1ji6| PDB=1ji6 | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS'''
| + | ===CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS=== |
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| - | ==Overview==
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| - | The coleopteran-active delta-endotoxin Cry3Bb1 from Bacillus thuringiensis (Bt) strain EG7231 is uniquely toxic to Diabrotica undecimpunctata, the Southern corn rootworm, while retaining activity against Leptinotarsa decemlineata, the Colorado potato beetle. The crystal structure of the delta-endotoxin Cry3Bb1 has been refined using data collected to 2.4 A resolution, with a residual R factor of 17.5% and an R(free) of 25.3%. The structure is made up of three domains: I, a seven-helix bundle (residues 64-294); II, a three-sheet domain (residues 295-502); and III, a beta-sandwich domain (residues 503-652). The monomers in the orthorhombic C222(1) crystal lattice form a dimeric quaternary structure across a crystallographic twofold axis, with a channel formed involving interactions between domains I and III. There are 23 hydrogen bonds between the two monomers conferring structural stability on the dimer. It has been demonstrated that Cry3Bb1 and the similar toxin Cry3A form oligomers in solution. The structural results presented here indicate that the interactions between domains I and III could be responsible for the initial higher order structure and have implications for the biological activity of these toxins. There are seven additional single amino-acid residues in the sequence of Cry3Bb1 compared with that of Cry3A; one in domain I, two in domain II and four in domain III, which also shows the largest conformational difference between the two proteins. These changes can be implicated in the selectivity differences noted for these two delta-endotoxins. | + | The line below this paragraph, {{ABSTRACT_PUBMED_11468393}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11468393 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11468393}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Cody, V.]] | | [[Category: Cody, V.]] |
| | [[Category: Cry3bb1]] | | [[Category: Cry3bb1]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:15:11 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:17:02 2008'' |
Revision as of 17:17, 1 July 2008
Template:STRUCTURE 1ji6
CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS
Template:ABSTRACT PUBMED 11468393
About this Structure
1JI6 is a Single protein structure of sequence from Bacillus thuringiensis. Full crystallographic information is available from OCA.
Reference
Structure of the insecticidal bacterial delta-endotoxin Cry3Bb1 of Bacillus thuringiensis., Galitsky N, Cody V, Wojtczak A, Ghosh D, Luft JR, Pangborn W, English L, Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1101-9. Epub 2001, Jul 23. PMID:11468393
Page seeded by OCA on Tue Jul 1 20:17:02 2008
