1jit

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{{STRUCTURE_1jit| PDB=1jit | SCENE= }}
{{STRUCTURE_1jit| PDB=1jit | SCENE= }}
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'''CRYSTAL STRUCTURE OF TETRAGONAL LYSOZYME GROWN IN PRESENCE 30% TREHALOSE'''
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===CRYSTAL STRUCTURE OF TETRAGONAL LYSOZYME GROWN IN PRESENCE 30% TREHALOSE===
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==Overview==
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In order to elucidate the effect of stabilizing additives on the structure of proteins and the associated ordered water molecules in the hydration shell, the crystal structures of tetragonal lysozyme grown in the presence of sucrose, sorbitol and trehalose have been refined. Also refined are the structures of orthorhombic and monoclinic lysozyme grown under the conditions in which tetragonal lysozyme is normally grown. A comparison of the two sets of structures with the structure of native tetragonal lysozyme shows that the effect of the additives on the structure of the protein molecule is less than that of the normal minor changes associated with differences in molecular packing. Surprisingly, the same is true of the effect on the hydration shell, represented by the ordered water molecules attached to the protein. Thus, it appears that the cause of the stabilizing effect of the additives needs to be sought outside the immediate neighbourhood of the protein molecule. Sorbitol and trehalose do not coherently interact with the protein. One sucrose molecule binds at the active-site cleft of the enzyme.
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The line below this paragraph, {{ABSTRACT_PUBMED_11679726}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11679726 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11679726}}
==About this Structure==
==About this Structure==
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[[Category: Hen egg-white lysozyme]]
[[Category: Hen egg-white lysozyme]]
[[Category: Mucopeptide n-acetylmuramyl hydrolase]]
[[Category: Mucopeptide n-acetylmuramyl hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:16:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:18:45 2008''

Revision as of 17:18, 1 July 2008

Image:1jit.png

Template:STRUCTURE 1jit

CRYSTAL STRUCTURE OF TETRAGONAL LYSOZYME GROWN IN PRESENCE 30% TREHALOSE

Template:ABSTRACT PUBMED 11679726

About this Structure

1JIT is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

The effect of stabilizing additives on the structure and hydration of proteins: a study involving tetragonal lysozyme., Datta S, Biswal BK, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1614-20. Epub 2001, Oct 25. PMID:11679726

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