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1jjk

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{{STRUCTURE_1jjk| PDB=1jjk | SCENE= }}
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'''Selenomethionine Substitution of Orotidine-5'-monophosphate Decarboxylase from E. coli Causes a Change in Crystal Contacts and Space Group'''
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===Selenomethionine Substitution of Orotidine-5'-monophosphate Decarboxylase from E. coli Causes a Change in Crystal Contacts and Space Group===
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==Overview==
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Orotidine 5'-monophosphate decarboxylase (ODCase) catalyses the decarboxylation of orotidine 5'-monophosphate to uridine 5'-monophosphate, the last step in the de novo biosynthesis of uridine 5'-monophosphate. In order to determine the structure of ODCase from Escherichia coli by the multi-wavelength anomalous dispersion technique, both native and SeMet-substituted proteins have been produced and purified. During the production of SeMet ODCase, it was observed that SeMet was the only amino acid that it was necessary to add to the defined medium during expression. SeMet-substituted ODCase in complex with the inhibitor 1-(5'-phospho-beta-D-ribofuranosyl)barbituric acid crystallizes under similar conditions as the native enzyme. In contrast to the native enzyme, where the crystals belong to the orthorhombic space group P2(1)2(1)2(1), the SeMet-substituted enzyme crystallizes in the monoclinic space group P2(1), with a quadrupling of the volume of the asymmetric unit. Despite the drastic difference in symmetry, the overall crystal packing is effectively identical in the two crystal forms. The change in space group appears to originate in differences in the crystal contacts near the SeMet and Met residues. These differences can be rationalized in terms of SeMet's larger size and hydrophobicity.
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(as it appears on PubMed at http://www.pubmed.gov), where 11526316 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11526316}}
==About this Structure==
==About this Structure==
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[[Category: Homodimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:20:50 2008''

Revision as of 17:20, 1 July 2008

Image:1jjk.png

Template:STRUCTURE 1jjk

Selenomethionine Substitution of Orotidine-5'-monophosphate Decarboxylase from E. coli Causes a Change in Crystal Contacts and Space Group

Template:ABSTRACT PUBMED 11526316

About this Structure

1JJK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Selenomethionine substitution of orotidine-5'-monophosphate decarboxylase causes a change in crystal contacts and space group., Poulsen JC, Harris P, Jensen KF, Larsen S, Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1251-9. Epub 2001, Aug 23. PMID:11526316

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