1jo8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1jo8.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1jo8.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1jo8| PDB=1jo8 | SCENE= }}
{{STRUCTURE_1jo8| PDB=1jo8 | SCENE= }}
-
'''Structural analysis of the yeast actin binding protein Abp1 SH3 domain'''
+
===Structural analysis of the yeast actin binding protein Abp1 SH3 domain===
-
==Overview==
+
<!--
-
Abp1p is an actin-binding protein that plays a central role in the organization of Saccharomyces cerevisiae actin cytoskeleton. By a combination of two-hybrid and phage-display approaches, we have identified six new ligands of the Abp1-SH3 domain. None of these SH3-mediated novel interactions was detected in recent all genome high throughput protein interaction projects. Here we show that the SH3-mediated association of Abp1p with the Ser/Thr kinases Prk1p and Ark1p is essential for their localization to actin cortical patches. The Abp1-SH3 domain has a rather unusual binding specificity, because its target peptides contain the tetrapentapeptide +XXXPXXPX+PXXL with positive charges flanking the polyproline core on both sides. Here we present the structure of the Abp1-SH3 domain solved at 1.3-A resolution. The peptide-binding pockets in the SH3 domain are flanked by two acidic residues that are uncommon at those positions in the SH3 domain family. We have shown by site-directed mutagenesis that one of these negatively charged side chains may be the key determinant for the preference for non-classical ligands.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11668184}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11668184 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11668184}}
==About this Structure==
==About this Structure==
Line 33: Line 37:
[[Category: Zucconi, A.]]
[[Category: Zucconi, A.]]
[[Category: Sh3 domain actin-binding-protein]]
[[Category: Sh3 domain actin-binding-protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:29:48 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:32:52 2008''

Revision as of 17:32, 1 July 2008

Image:1jo8.png

Template:STRUCTURE 1jo8

Structural analysis of the yeast actin binding protein Abp1 SH3 domain

Template:ABSTRACT PUBMED 11668184

About this Structure

1JO8 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Unusual binding properties of the SH3 domain of the yeast actin-binding protein Abp1: structural and functional analysis., Fazi B, Cope MJ, Douangamath A, Ferracuti S, Schirwitz K, Zucconi A, Drubin DG, Wilmanns M, Cesareni G, Castagnoli L, J Biol Chem. 2002 Feb 15;277(7):5290-8. Epub 2001 Oct 19. PMID:11668184

Page seeded by OCA on Tue Jul 1 20:32:52 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jo8"
Personal tools