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1jp5

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{{STRUCTURE_1jp5| PDB=1jp5 | SCENE= }}
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'''Crystal structure of the single-chain Fv fragment 1696 in complex with the epitope peptide corresponding to N-terminus of HIV-1 protease'''
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===Crystal structure of the single-chain Fv fragment 1696 in complex with the epitope peptide corresponding to N-terminus of HIV-1 protease===
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==Overview==
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BACKGROUND: Since the demonstration that the protease of the human immunodeficiency virus (HIV Pr) is essential in the viral life cycle, this enzyme has become one of the primary targets for antiviral drug design. The murine monoclonal antibody 1696 (mAb1696), produced by immunization with the HIV-1 protease, inhibits the catalytic activity of the enzyme of both the HIV-1 and HIV-2 isolates with inhibition constants in the low nanomolar range. The antibody cross-reacts with peptides that include the N terminus of the enzyme, a region that is highly conserved in sequence among different viral strains and that, furthermore, is crucial for homodimerization to the active enzymatic form. RESULTS: We report here the crystal structure at 2.7 A resolution of a recombinant single-chain Fv fragment of mAb1696 as a complex with a cross-reactive peptide of the HIV-1 protease. The antibody-antigen interactions observed in this complex provide a structural basis for understanding the origin of the broad reactivity of mAb-1696 for the HIV-1 and HIV-2 proteases and their respective N-terminal peptides. CONCLUSION: A possible mechanism of HIV-protease inhibition by mAb1696 is proposed that could help the design of inhibitors aimed at binding inactive monomeric species.
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(as it appears on PubMed at http://www.pubmed.gov), where 11591344 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11591344}}
==About this Structure==
==About this Structure==
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[[Category: Antibody-antigen complex]]
[[Category: Antibody-antigen complex]]
[[Category: Hiv pr inhibiting antibody]]
[[Category: Hiv pr inhibiting antibody]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:32:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:34:59 2008''

Revision as of 17:35, 1 July 2008

Image:1jp5.png

Template:STRUCTURE 1jp5

Crystal structure of the single-chain Fv fragment 1696 in complex with the epitope peptide corresponding to N-terminus of HIV-1 protease

Template:ABSTRACT PUBMED 11591344

About this Structure

Full crystallographic information is available from OCA.

Reference

Structural basis of HIV-1 and HIV-2 protease inhibition by a monoclonal antibody., Rezacova P, Lescar J, Brynda J, Fabry M, Horejsi M, Sedlacek J, Bentley GA, Structure. 2001 Oct;9(10):887-95. PMID:11591344

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