1hhv
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(New page: 200px<br /><applet load="1hhv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hhv" /> '''SOLUTION STRUCTURE OF VIRUS CHEMOKINE VMIP-I...)
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Revision as of 14:28, 20 November 2007
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SOLUTION STRUCTURE OF VIRUS CHEMOKINE VMIP-II
Overview
Human herpesvirus-8 (HHV-8) is the infectious agent responsible for, Kaposi's sarcoma and encodes a protein, macrophage inflammatory protein-II, (vMIP-II), which shows sequence similarity to the human CC chemokines., vMIP-II has broad receptor specificity that crosses chemokine receptor, subfamilies, and inhibits HIV-1 viral entry mediated by numerous chemokine, receptors. In this study, the solution structure of chemically synthesized, vMIP-II was determined by nuclear magnetic resonance. The protein is a, monomer and possesses the chemokine fold consisting of a flexible, N-terminus, three antiparallel beta strands, and a C-terminal alpha helix., Except for the N-terminal residues (residues 1-13) and the last two, C-terminal residues (residues 73-74), the structure of vMIP-II is, well-defined, exhibiting average rmsd of 0.35 and 0.90 A for the backbone, heavy atoms and all heavy atoms of residues 14-72, respectively. Taking, into account the sequence differences between the various CC chemokines, and comparing their three-dimensional structures allows us to implicate, residues that influence the quaternary structure and receptor binding and, activation of these proteins in solution. The analysis of the sequence and, three-dimensional structure of vMIP-II indicates the presence of epitopes, involved in binding two receptors CCR2 and CCR5. We propose that vMIP-II, was initially specific for CCR5 and acquired receptor-binding properties, to CCR2 and other chemokine receptors.
About this Structure
1HHV is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
CCR2 and CCR5 receptor-binding properties of herpesvirus-8 vMIP-II based on sequence analysis and its solution structure., Shao W, Fernandez E, Sachpatzidis A, Wilken J, Thompson DA, Schweitzer BI, Lolis E, Eur J Biochem. 2001 May;268(10):2948-59. PMID:11358512
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