This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1jtv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1jtv.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1jtv.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1jtv| PDB=1jtv | SCENE= }}
{{STRUCTURE_1jtv| PDB=1jtv | SCENE= }}
-
'''Crystal structure of 17beta-Hydroxysteroid Dehydrogenase Type 1 complexed with Testosterone'''
+
===Crystal structure of 17beta-Hydroxysteroid Dehydrogenase Type 1 complexed with Testosterone===
-
==Overview==
+
<!--
-
Steroids are implicated in many physiological processes, such as reproduction, aging, metabolism, and cancer. To understand the molecular basis for steroid recognition and discrimination, we studied the human estrogenic 17beta-hydroxysteroid dehydrogenase (17beta-HSD1) responsible for the last step in the bioactivation of all estrogens. Here we report the first observation of the conversion of dihydrotestosterone (DHT) into 3beta,17beta-androstanediol (3beta-diol) by 17beta-HSD1, an estrogenic enzyme studied for more than half a century. Kinetic observations demonstrate that both the 3beta-reduction of DHT into 3beta-diol (kcat = 0.040 s(-1)1; Km = 32 +/- 9 microM) and the 17beta-oxidation of DHT into androstandione (A-dione) (kcat = 0.19 s(-1); Km = 26 +/-6 microM) are catalyzed by 17beta-HSD1 via alternative binding orientation of the steroid. The reduction of DHT was also observed in intact cells by using HEK-293 cells stably transformed with 17beta-HSD1. The high-resolution structure of a 17beta-HSD1-C19-steroid (testosterone) complex solved at 1.54 A demonstrates that the steroid is reversibly oriented in the active site, which strongly supports the existence of alternative binding mode. Such a phenomenon can be explained by the pseudo-symmetric structure of C19-steroids. Our results confirm the role of the Leu149 residue in C18/C19-steroid discrimination and suggest a possible mechanism of 17beta-HSD1 in the modulation of DHT levels in tissues, such as the breast, where both the enzyme and DHT are present.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12490543}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12490543 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12490543}}
==About this Structure==
==About this Structure==
Line 28: Line 32:
[[Category: Alternative binding mode]]
[[Category: Alternative binding mode]]
[[Category: Steroid hormone]]
[[Category: Steroid hormone]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:54:51 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:50:50 2008''

Revision as of 17:50, 1 July 2008

Image:1jtv.png

Template:STRUCTURE 1jtv

Crystal structure of 17beta-Hydroxysteroid Dehydrogenase Type 1 complexed with Testosterone

Template:ABSTRACT PUBMED 12490543

About this Structure

1JTV is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Pseudo-symmetry of C19 steroids, alternative binding orientations, and multispecificity in human estrogenic 17beta-hydroxysteroid dehydrogenase., Gangloff A, Shi R, Nahoum V, Lin SX, FASEB J. 2003 Feb;17(2):274-6. Epub 2002 Dec 17. PMID:12490543

Page seeded by OCA on Tue Jul 1 20:50:50 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jtv"
Personal tools