1ju3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1ju3.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1ju3.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1ju3| PDB=1ju3 | SCENE= }}
{{STRUCTURE_1ju3| PDB=1ju3 | SCENE= }}
-
'''BACTERIAL COCAINE ESTERASE COMPLEX WITH TRANSITION STATE ANALOG'''
+
===BACTERIAL COCAINE ESTERASE COMPLEX WITH TRANSITION STATE ANALOG===
-
==Overview==
+
<!--
-
Here we report the first structure of a cocaine-degrading enzyme. The bacterial esterase, cocE, hydrolyzes pharmacologically active (-)-cocaine to a non-psychoactive metabolite with a rate faster than any other reported cocaine esterase (kcat = 7.8 s-1 and KM = 640 nM). Because of the high catalytic proficiency of cocE, it is an attractive candidate for novel protein-based therapies for cocaine overdose. The crystal structure of cocE, solved by multiple anomalous dispersion (MAD) methods, reveals that cocE is a serine esterase composed of three domains: (i) a canonical alpha/beta hydrolase fold (ii) an alpha-helical domain that caps the active site and (iii) a jelly-roll-like beta-domain that interacts extensively with the other two domains. The active site was identified within the interface of all three domains by analysis of the crystal structures of transition state analog adduct and product complexes, which were refined at 1.58 A and 1.63 A resolution, respectively. These structural studies suggest that substrate recognition arises partly from interactions between the benzoyl moiety of cocaine and a highly evolved specificity pocket.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11742345}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11742345 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11742345}}
==About this Structure==
==About this Structure==
Line 31: Line 35:
[[Category: Wilson, I A.]]
[[Category: Wilson, I A.]]
[[Category: Alpha/beta hydrolase]]
[[Category: Alpha/beta hydrolase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:55:21 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:52:16 2008''

Revision as of 17:52, 1 July 2008

Image:1ju3.png

Template:STRUCTURE 1ju3

BACTERIAL COCAINE ESTERASE COMPLEX WITH TRANSITION STATE ANALOG

Template:ABSTRACT PUBMED 11742345

About this Structure

1JU3 is a Single protein structure of sequence from Rhodococcus sp. mb1. Full crystallographic information is available from OCA.

Reference

Crystal structure of a bacterial cocaine esterase., Larsen NA, Turner JM, Stevens J, Rosser SJ, Basran A, Lerner RA, Bruce NC, Wilson IA, Nat Struct Biol. 2002 Jan;9(1):17-21. PMID:11742345

Page seeded by OCA on Tue Jul 1 20:52:16 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ju3"
Personal tools