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1jud

From Proteopedia

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{{STRUCTURE_1jud| PDB=1jud | SCENE= }}
{{STRUCTURE_1jud| PDB=1jud | SCENE= }}
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'''L-2-HALOACID DEHALOGENASE'''
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===L-2-HALOACID DEHALOGENASE===
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==Overview==
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L-2-Haloacid dehalogenase catalyzes the hydrolytic dehalogenation of L-2-haloalkanoic acids to yield the corresponding D-2-hydroxyalkanoic acids. The crystal structure of the homodimeric enzyme from Pseudomonas sp. YL has been determined by a multiple isomorphous replacement method and refined at 2.5 A resolution to a crystallographic R-factor of 19.5%. The subunit consists of two structurally distinct domains: the core domain and the subdomain. The core domain has an alpha/beta structure formed by a six-stranded parallel beta-sheet flanked by five alpha-helices. The subdomain inserted into the core domain has a four helix bundle structure providing the greater part of the interface for dimer formation. There is an active site cavity between the domains. An experimentally identified nucleophilic residue, Asp-10, is located on a loop following the amino-terminal beta-strand in the core domain, and other functional residues, Thr-14, Arg-41, Ser-118, Lys-151, Tyr-157, Ser-175, Asn-177, and Asp-180, detected by a site-directed mutagenesis experiment, are arranged around the nucleophile in the active site. Although the enzyme is an alpha/beta-type hydrolase, it does not belong to the alpha/beta hydrolase fold family, from the viewpoint of the topological feature and the position of the nucleophile.
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(as it appears on PubMed at http://www.pubmed.gov), where 8702766 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8702766}}
==About this Structure==
==About this Structure==
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[[Category: Dehalogenase]]
[[Category: Dehalogenase]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:56:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:53:39 2008''

Revision as of 17:53, 1 July 2008

Image:1jud.png

Template:STRUCTURE 1jud

L-2-HALOACID DEHALOGENASE

Template:ABSTRACT PUBMED 8702766

About this Structure

1JUD is a Single protein structure of sequence from Pseudomonas. Full crystallographic information is available from OCA.

Reference

Crystal structure of L-2-haloacid dehalogenase from Pseudomonas sp. YL. An alpha/beta hydrolase structure that is different from the alpha/beta hydrolase fold., Hisano T, Hata Y, Fujii T, Liu JQ, Kurihara T, Esaki N, Soda K, J Biol Chem. 1996 Aug 23;271(34):20322-30. PMID:8702766

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