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1hji
From Proteopedia
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(New page: 200px<br /><applet load="1hji" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hji" /> '''BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA ...)
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Revision as of 14:29, 20 November 2007
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BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX
Overview
Nun protein from coliphage HK022 binds to phage boxB RNA and functions, in, contrast to phage lambda N protein, as a transcriptional terminator. The, basic Nun-(10-44) peptide contains the boxB RNA binding arginine rich, motif, ARM. The peptide binds boxB RNA and competes with the phage lambda, ARM peptide N-(1-36) as indicated by nuclear magnetic resonance (NMR), spectroscopy titrations. In two-dimensional nuclear Overhauser enhancement, spectroscopy experiments boxB RNA in complex with Nun-(20-44) exhibits the, same pattern of resonances as it does in complex with N peptides, containing the ARM, and we could show that Nun-(20-44) forms a bent, alpha-helix upon binding to the boxB RNA. The structure of the boxB, RNA-bound Nun-(20-44) was determined on the basis of 191 intra- and 30, intermolecular distance restraints. Ser-24 is anchored to the lower RNA, stem, and stacking of Tyr-39 and A7 is clearly experimentally indicated., Arg-28 shows numerous contacts to the RNA stem. Leu-22, Ile-30, Trp-33, Ile-37, and Leu-41 form a hydrophobic surface, which could be a, recognition site for additional host factors such as NusG. Such a, hydrophobic surface area is not present in N-(1-36) bound to boxB RNA.
About this Structure
1HJI is a Protein complex structure of sequences from Enterobacteria phage hk022. Full crystallographic information is available from OCA.
Reference
The structure of the coliphage HK022 Nun protein-lambda-phage boxB RNA complex. Implications for the mechanism of transcription termination., Faber C, Scharpf M, Becker T, Sticht H, Rosch P, J Biol Chem. 2001 Aug 24;276(34):32064-70. Epub 2001 May 16. PMID:11356847
Page seeded by OCA on Tue Nov 20 16:36:38 2007
