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1jv5

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[[Image:1jv5.jpg|left|200px]]
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{{STRUCTURE_1jv5| PDB=1jv5 | SCENE= }}
{{STRUCTURE_1jv5| PDB=1jv5 | SCENE= }}
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'''Anti-blood group A Fv'''
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===Anti-blood group A Fv===
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==Overview==
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The specificity of antibody recognition of the ABO blood group trisaccharide antigens has been explored by crystal structure analysis and mutation methods. The crystal structure of the Fv corresponding to the anti-blood group A antibody AC1001 has been determined to 2.2-A resolution and reveals a binding pocket that is complementary to the blood group A-trisaccharide antigen. The effect of mutating specific residues lining this pocket on binding to the A and B blood group oligosaccharide antigens was investigated through a panel of single point mutations and through a phage library of mutations in complementarity determining region H3. Both approaches gave several mutants with improved affinity for antigen. Surface plasmon resonance indicated up to 8-fold enhancement in affinity for the A-pentasaccharide with no observable binding to the blood group B antigen. This is the first example of single point mutations in a carbohydrate-binding antibody resulting in significant increases in binding affinity without loss of specificity.
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(as it appears on PubMed at http://www.pubmed.gov), where 11679577 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11679577}}
==About this Structure==
==About this Structure==
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[[Category: Young, N M.]]
[[Category: Young, N M.]]
[[Category: Immunoglobulin]]
[[Category: Immunoglobulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:57:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:57:47 2008''

Revision as of 17:57, 1 July 2008

Image:1jv5.png

Template:STRUCTURE 1jv5

Anti-blood group A Fv

Template:ABSTRACT PUBMED 11679577

About this Structure

1JV5 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of an anti-blood group A Fv and improvement of its binding affinity without loss of specificity., Thomas R, Patenaude SI, MacKenzie CR, To R, Hirama T, Young NM, Evans SV, J Biol Chem. 2002 Jan 18;277(3):2059-64. Epub 2001 Oct 25. PMID:11679577

Page seeded by OCA on Tue Jul 1 20:57:47 2008

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