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| - | [[Image:1jvb.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1jvb| PDB=1jvb | SCENE= }} | | {{STRUCTURE_1jvb| PDB=1jvb | SCENE= }} |
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| - | '''ALCOHOL DEHYDROGENASE FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS'''
| + | ===ALCOHOL DEHYDROGENASE FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS=== |
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| - | ==Overview==
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| - | The crystal structure of a medium-chain NAD(H)-dependent alcohol dehydrogenase (ADH) from an archaeon has been solved by multiwavelength anomalous diffraction, using a selenomethionine-substituted enzyme. The protein (SsADH), extracted from the hyperthermophilic organism Sulfolobus solfataricus, is a homo-tetramer with a crystallographic 222 symmetry. Despite the low level of sequence identity, the overall fold of the monomer is similar to that of the other homologous ADHs of known structure. However, a significant difference is the orientation of the catalytic domain relative to the coenzyme-binding domain that results in a larger interdomain cleft. At the bottom of this cleft, the catalytic zinc ion is coordinated tetrahedrally and lacks the zinc-bound water molecule that is usually found in ADH apoform structures. The fourth coordination position is indeed occupied by a Glu residue, as found in bacterial tetrameric ADHs. Other differences are found in the architecture of the substrate pocket whose entrance is more restricted than in other ADHs. SsADH is the first tetrameric ADH X-ray structure containing a second zinc ion playing a structural role. This latter metal ion shows a peculiar coordination, with a glutamic acid residue replacing one of the four cysteine ligands that are highly conserved throughout the structural zinc-containing dimeric ADHs.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_12051852}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12051852 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12051852}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Nad]] | | [[Category: Nad]] |
| | [[Category: Zinc]] | | [[Category: Zinc]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:58:14 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:58:55 2008'' |
Revision as of 17:59, 1 July 2008
Template:STRUCTURE 1jvb
ALCOHOL DEHYDROGENASE FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS
Template:ABSTRACT PUBMED 12051852
About this Structure
1JVB is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the alcohol dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus at 1.85 A resolution., Esposito L, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A, J Mol Biol. 2002 Apr 26;318(2):463-77. PMID:12051852
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