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| | {{STRUCTURE_1jve| PDB=1jve | SCENE= }} | | {{STRUCTURE_1jve| PDB=1jve | SCENE= }} |
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| - | '''NMR Structure of an AT-Rich DNA with the GAA-Hairpin Loop'''
| + | ===NMR Structure of an AT-Rich DNA with the GAA-Hairpin Loop=== |
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| - | ==Overview==
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| - | We have determined, by proton NMR and complete relaxation matrix methods, the high-resolution structure of a DNA oligonucleotide in solution with nine contiguous AT base pairs. The stretch of AT pairs, TAATTATAA x TTATAATTA, is imbedded in a 27-nucleotide stem-and-loop construct, which is stabilized by terminal GC base pairs and an extraordinarily stable DNA loop GAA (Hirao et al., 1994, Nucleic Acids Res. 22, 576-582). The AT-rich sequence has three repeated TAA x TTA motifs, one in the reverse orientation. Comparison of the local conformations of the three motifs shows that the sequence context has a minor effect here: atomic RMSD between the three TAA x TTA fragments is 0.4-0.5 A, while each fragment is defined within the RMSD of 0.3-0.4 A. The AT-rich stem also contains a consensus sequence for the Pribnow box, TATAAT. The TpA, ApT, and TpT x ApA steps have characteristic local conformations, a combination of which determines a unique sequence-dependent pattern of minor groove width variation. All three TpA steps are locally bent in the direction compressing the major groove of DNA. These bends, however, compensate each other, because of their relative position in the sequence, so that the overall helical axis is essentially straight.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11991355}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11991355 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11991355}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JVE OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JVE OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Pribnow box]] | | [[Category: Pribnow box]] |
| | [[Category: Stem-and-loop]] | | [[Category: Stem-and-loop]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:58:28 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:59:13 2008'' |
Revision as of 17:59, 1 July 2008
Template:STRUCTURE 1jve
NMR Structure of an AT-Rich DNA with the GAA-Hairpin Loop
Template:ABSTRACT PUBMED 11991355
About this Structure
Full experimental information is available from OCA.
Reference
High-resolution NMR structure of an AT-rich DNA sequence., Ulyanov NB, Bauer WR, James TL, J Biomol NMR. 2002 Mar;22(3):265-80. PMID:11991355
Page seeded by OCA on Tue Jul 1 20:59:13 2008
