1jvy

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[[Image:1jvy.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1jvy| PDB=1jvy | SCENE= }}
{{STRUCTURE_1jvy| PDB=1jvy | SCENE= }}
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'''Maltodextrin-binding protein variant D207C/A301GS/P316C with beta-mercaptoethanol mixed disulfides'''
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===Maltodextrin-binding protein variant D207C/A301GS/P316C with beta-mercaptoethanol mixed disulfides===
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==Overview==
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Cysteine substitutions were engineered on the surface of maltose binding protein to produce crystine fibers, linear polymers of folded protein formed within a crystal. Disulfide bond formation between adjacent protein molecules within the lattice was monitored by X-ray crystallography. The cross-linked crystals were resistant to dissolution in water or neutral buffer solutions, even though the cross-linking was one-dimensional. However, crystine fibers were observed by transmission electron microscopy to dissociate from the crystals in acidic solutions. Some fibers remained associated as two-dimensional bundles or sheets, with a repeat unit along the fibers consistent with the packing of the individual protein molecules in the crystal. Neutralization of the acidic solutions caused the fibers to re-associate as a solid. Crystine threads were drawn out of this solution. In scanning electron microscopy images, many individual fibers could be seen unwinding from the ends of some threads. Crystine fibers are a new type of biomolecular material with potential applications wherever the use of proteins in a fibrous form is desirable, for example, the incorporation of enzymes into cloth or filtration material.
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(as it appears on PubMed at http://www.pubmed.gov), where 12538909 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12538909}}
==About this Structure==
==About this Structure==
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[[Category: Disulfide]]
[[Category: Disulfide]]
[[Category: Intermolecular]]
[[Category: Intermolecular]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:59:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 21:02:42 2008''

Revision as of 18:02, 1 July 2008

Image:1jvy.png

Template:STRUCTURE 1jvy

Maltodextrin-binding protein variant D207C/A301GS/P316C with beta-mercaptoethanol mixed disulfides

Template:ABSTRACT PUBMED 12538909

About this Structure

1JVY is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystine: fibrous biomolecular material from protein crystals cross-linked in a specific geometry., Srinivasan U, Iyer GH, Przybycien TA, Samsonoff WA, Bell JA, Protein Eng. 2002 Nov;15(11):895-902. PMID:12538909

Page seeded by OCA on Tue Jul 1 21:02:42 2008

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