1jyk

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{{STRUCTURE_1jyk| PDB=1jyk | SCENE= }}
{{STRUCTURE_1jyk| PDB=1jyk | SCENE= }}
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'''Catalytic Mechanism of CTP:phosphocholine Cytidylytransferase from Streptococcus pneumoniae (LicC)'''
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===Catalytic Mechanism of CTP:phosphocholine Cytidylytransferase from Streptococcus pneumoniae (LicC)===
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==Overview==
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Pneumococcal LicC is a member of the nucleoside triphosphate transferase superfamily and catalyzes the transfer of a cytidine monophosphate from CTP to phosphocholine to form CDP-choline. The structures of apo-LicC and the LicC-CDP-choline-Mg(2+) ternary complex were determined, and the comparison of these structures reveals a significant conformational change driven by the multivalent coordination of Mg(2+). The key event is breaking the Glu(216)-Arg(129) salt bridge, which triggers the coalescence of four individual beta-strands into two extended beta-sheets. These movements reorient the side chains of Trp(136) and Tyr(190) for the optimal binding and alignment of the phosphocholine moiety. Consistent with these conformational changes, LicC operates via a compulsory ordered kinetic mechanism. The structures explain the substrate specificity of LicC for CTP and phosphocholine and implicate a direct role for Mg(2+) in aligning phosphocholine for in-line nucleophilic attack and stabilizing the negative charge that develops in the pentacoordinate transition state. These results provide a structural basis for assigning a specific role for magnesium in the catalytic mechanism of pneumococcal LicC.
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The line below this paragraph, {{ABSTRACT_PUBMED_11706035}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11706035 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11706035}}
==About this Structure==
==About this Structure==
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[[Category: Ctp:phosphocholine cytidylyltransferase]]
[[Category: Ctp:phosphocholine cytidylyltransferase]]
[[Category: Licc]]
[[Category: Licc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:04:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 21:07:11 2008''

Revision as of 18:07, 1 July 2008

Image:1jyk.png

Template:STRUCTURE 1jyk

Catalytic Mechanism of CTP:phosphocholine Cytidylytransferase from Streptococcus pneumoniae (LicC)

Template:ABSTRACT PUBMED 11706035

About this Structure

1JYK is a Single protein structure of sequence from Streptococcus pneumoniae. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of CTP:phosphocholine cytidylyltransferase (LicC) from Streptococcus pneumoniae., Kwak BY, Zhang YM, Yun M, Heath RJ, Rock CO, Jackowski S, Park HW, J Biol Chem. 2002 Feb 8;277(6):4343-50. Epub 2001 Nov 12. PMID:11706035

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