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1k19

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{{STRUCTURE_1k19| PDB=1k19 | SCENE= }}
{{STRUCTURE_1k19| PDB=1k19 | SCENE= }}
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'''NMR Solution Structure of the Chemosensory Protein CSP2 from Moth Mamestra brassicae'''
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===NMR Solution Structure of the Chemosensory Protein CSP2 from Moth Mamestra brassicae===
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==Overview==
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Chemosensory proteins (CSPs) are believed to be involved in chemical communication and perception. A number of such proteins, of molecular mass approximately 13 kDa, have been isolated from different sensory organs of a wide range of insect species. Several CSPs have been identified in the antennae and proboscis of the moth Mamestra brassicae. CSPMbraA6, a 112-amino-acid antennal protein, has been expressed in a soluble form in large quantities in the Escherichi coli periplasm. NMR structure determination of CSPMbraA6 has been performed with 1H- and 15N-labelled samples. The calculated structures present an average root mean square deviation about the mean structure of 0.63 A for backbone atoms and 1.27 A for all non-hydrogen atoms except the 12 N-terminal residues. The protein is well folded from residue 12 to residue 110, and consists of a non-bundle alpha-helical structure with six helices connected by alpha alpha loops. It has a globular shape, with overall dimensions of 32 A x 28 A x 24 A. A channel is visible in the hydrophobic core, with dimensions of 3 A x 9 A x 21 A. In some of the 20 solution structures calculated, this channel is closed either by Trp-94 at one end or by Tyr-26 at the other end; in some other solutions, this channel is closed at both ends. Binding experiments with 12-bromododecanol indicate that the CSPMbraA6 structure is modified upon ligand binding.
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{{ABSTRACT_PUBMED_12217077}}
==About this Structure==
==About this Structure==
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1K19 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mamestra_brassicae Mamestra brassicae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K19 OCA].
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1K19 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mamestra_brassicae Mamestra brassicae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K19 OCA].
==Reference==
==Reference==
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[[Category: Nmr]]
[[Category: Nmr]]
[[Category: Pheromone]]
[[Category: Pheromone]]
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Revision as of 18:15, 1 July 2008

Template:STRUCTURE 1k19

NMR Solution Structure of the Chemosensory Protein CSP2 from Moth Mamestra brassicae

Template:ABSTRACT PUBMED 12217077

About this Structure

1K19 is a Single protein structure of sequence from Mamestra brassicae. Full experimental information is available from OCA.

Reference

Solution structure of a chemosensory protein from the moth Mamestra brassicae., Mosbah A, Campanacci V, Lartigue A, Tegoni M, Cambillau C, Darbon H, Biochem J. 2003 Jan 1;369(Pt 1):39-44. PMID:12217077

Page seeded by OCA on Tue Jul 1 21:15:29 2008

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