1k87

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{{STRUCTURE_1k87| PDB=1k87 | SCENE= }}
{{STRUCTURE_1k87| PDB=1k87 | SCENE= }}
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'''Crystal structure of E.coli PutA (residues 1-669)'''
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===Crystal structure of E.coli PutA (residues 1-669)===
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==Overview==
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The PutA flavoprotein from Escherichia coli plays multiple roles in proline catabolism by functioning as a membrane-associated bi-functional enzyme and a transcriptional repressor of proline utilization genes. The human homolog of the PutA proline dehydrogenase (PRODH) domain is critical in p53-mediated apoptosis and schizophrenia. Here we report the crystal structure of a 669-residue truncated form of PutA that shows both PRODH and DNA-binding activities, representing the first structure of a PutA protein and a PRODH enzyme from any organism. The structure is a domain-swapped dimer with each subunit comprising three domains: a helical dimerization arm, a 120-residue domain containing a three-helix bundle similar to that in the helix-turn-helix superfamily of DNA-binding proteins and a beta/alpha-barrel PRODH domain with a bound lactate inhibitor. Analysis of the structure provides insight into the mechanism of proline oxidation to pyrroline-5-carboxylate, and functional studies of a mutant protein suggest that the DNA-binding domain is located within the N-terminal 261 residues of E. coli PutA.
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The line below this paragraph, {{ABSTRACT_PUBMED_12514740}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12514740 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12514740}}
==About this Structure==
==About this Structure==
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[[Category: Shuttling]]
[[Category: Shuttling]]
[[Category: Transcriptional repressor]]
[[Category: Transcriptional repressor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:25:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 09:55:46 2008''

Revision as of 06:55, 2 July 2008

Image:1k87.png

Template:STRUCTURE 1k87

Crystal structure of E.coli PutA (residues 1-669)

Template:ABSTRACT PUBMED 12514740

About this Structure

1K87 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of the proline dehydrogenase domain of the multifunctional PutA flavoprotein., Lee YH, Nadaraia S, Gu D, Becker DF, Tanner JJ, Nat Struct Biol. 2003 Feb;10(2):109-14. PMID:12514740

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