This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1k9w

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1k9w.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1k9w.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1k9w| PDB=1k9w | SCENE= }}
{{STRUCTURE_1k9w| PDB=1k9w | SCENE= }}
-
'''HIV-1(MAL) RNA Dimerization Initiation Site'''
+
===HIV-1(MAL) RNA Dimerization Initiation Site===
-
==Overview==
+
<!--
-
We describe the crystal structures of the RNA dimerization initiation sites (DIS) of HIV-1 subtypes A and B. Both molecules adopt a hairpin conformation, with loop sequences consisting of 272-AGGUGCACA-280 and 272-AAGCGCGCA-280, respectively. This includes a six-base self-complementary stretch (underlined) that allows homodimerization through the formation of a loop-loop, or 'kissing-loop', complex. The DISs for the two sequences have identical conformations, and the two interacting hairpins show a perfect coaxial alignment. The conserved purines, A272 and R273, are stacked in a bulged-out conformation and symmetrically join the upward and downward strands of each hairpin by crossing the helix major groove. There is good agreement between these structures and previous results from chemical probing in solution, as well as with differences in magnesium dependence for dimerization. The overall shape of the kissing-loop complex is very similar to that of the previously determined subtype A DIS duplex form. Unexpectedly, the purine R273 is the only base seen at a different position and is responsible for the difference in topology between the two forms. We propose that the transition from kissing-loop duplex could occur by a recombination mechanism based on symmetrical chain cleavage at R273 of each hairpin and subsequent cross-religation, rather than by base-pair melting and subsequent reannealing.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11702070}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11702070 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11702070}}
==About this Structure==
==About this Structure==
Line 28: Line 32:
[[Category: Hiv]]
[[Category: Hiv]]
[[Category: Rna]]
[[Category: Rna]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:29:13 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:00:36 2008''

Revision as of 07:00, 2 July 2008

Image:1k9w.png

Template:STRUCTURE 1k9w

HIV-1(MAL) RNA Dimerization Initiation Site

Template:ABSTRACT PUBMED 11702070

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structures of coaxially stacked kissing complexes of the HIV-1 RNA dimerization initiation site., Ennifar E, Walter P, Ehresmann B, Ehresmann C, Dumas P, Nat Struct Biol. 2001 Dec;8(12):1064-8. PMID:11702070

Page seeded by OCA on Wed Jul 2 10:00:36 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1k9w"
Personal tools