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| - | [[Image:1kbu.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1kbu.png|left|200px]] |
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| | {{STRUCTURE_1kbu| PDB=1kbu | SCENE= }} | | {{STRUCTURE_1kbu| PDB=1kbu | SCENE= }} |
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| - | '''CRE RECOMBINASE BOUND TO A LOXP HOLLIDAY JUNCTION'''
| + | ===CRE RECOMBINASE BOUND TO A LOXP HOLLIDAY JUNCTION=== |
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| - | ==Overview==
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| - | Cre recombinase uses two pairs of sequential cleavage and religation reactions to exchange homologous DNA strands between 34 base-pair (bp) LoxP recognition sequences. In the oligomeric recombination complex, a switch between "cleaving" and "non-cleaving" subunit conformations regulates the number, order, and regio-specificity of the strand exchanges. However, the particular sequence of events has been in question. From analysis of strand composition of the Holliday junction (HJ) intermediate, we determined that Cre initiates recombination of LoxP by cleaving the upper strand on the left arm. Cre preferred to react with the left arm of a LoxP suicide substrate, but at a similar rate to the right arm, indicating that the first strand to be exchanged is selected prior to cleavage. We propose that during complex assembly the cleaving subunit preferentially associates with the LoxP left arm, directing the first strand exchange to that side. In addition, this biased assembly would enforce productive orientation of LoxP sites in the recombination synapses. A novel Cre-HJ complex structure in which LoxP was oriented with the left arm bound by the cleaving Cre subunit suggested a physical basis for the strand exchange order. Lys86 and Lys201 interact with the left arm scissile adenine base differently than in structures that have a scissile guanine. These interactions are associated with positioning the 198-208 loop, a structural component of the conformational switch, in a configuration that is specific to the cleaving conformation. Our results suggest that strand exchange order and site alignment are regulated by an "induced fit" mechanism in which the cleaving conformation is selectively stabilized through protein-DNA interactions with the scissile base on the strand that is cleaved first.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_12051940}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12051940 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12051940}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Int recombinase mechanism]] | | [[Category: Int recombinase mechanism]] |
| | [[Category: Site-specific recombinase]] | | [[Category: Site-specific recombinase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:33:11 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:06:06 2008'' |
Revision as of 07:06, 2 July 2008
Template:STRUCTURE 1kbu
CRE RECOMBINASE BOUND TO A LOXP HOLLIDAY JUNCTION
Template:ABSTRACT PUBMED 12051940
About this Structure
1KBU is a Protein complex structure of sequences from Enterobacteria phage p1. Full crystallographic information is available from OCA.
Reference
The order of strand exchanges in Cre-LoxP recombination and its basis suggested by the crystal structure of a Cre-LoxP Holliday junction complex., Martin SS, Pulido E, Chu VC, Lechner TS, Baldwin EP, J Mol Biol. 2002 May 24;319(1):107-27. PMID:12051940
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