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1kcb

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{{STRUCTURE_1kcb| PDB=1kcb | SCENE= }}
{{STRUCTURE_1kcb| PDB=1kcb | SCENE= }}
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'''Crystal Structure of a NO-forming Nitrite Reductase Mutant: an Analog of a Transition State in Enzymatic Reaction'''
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===Crystal Structure of a NO-forming Nitrite Reductase Mutant: an Analog of a Transition State in Enzymatic Reaction===
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==Overview==
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I257E was obtained by site directed mutagenesis of nitrite reductase from Achromobacter cycloclastes. The mutant has no enzyme activity. Its crystal structure determined at 1.65A resolution shows that the side-chain carboxyl group of the mutated residue, Glu257, coordinates with the type 2 copper in the mutant and blocks the contact between the type 2 copper and its solvent channel, indicating that the accessibility of the type 2 copper is essential for maintaining the activity of nitrite reductase. The carboxylate is an analog of the substrate, nitrite, but the distances between the type 2 copper and the two oxygen atoms of the side-chain carboxyl group are reversed in comparison to the binding of nitrite to the native enzyme. In the mutant, both the type 2 copper and the N epsilon atom on the imidazole ring of its coordinated residue His135 move in the substrate binding direction relative to the native enzyme. In addition, an EPR study showed that the type 2 copper in the mutant is in a reduced state. We propose that mutant I257E is in a state corresponding to a transition state in the enzymatic reaction.
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The line below this paragraph, {{ABSTRACT_PUBMED_12615072}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12615072 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12615072}}
==About this Structure==
==About this Structure==
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[[Category: Beta barrel]]
[[Category: Beta barrel]]
[[Category: Copper-containing nitrite reductase]]
[[Category: Copper-containing nitrite reductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:34:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:07:17 2008''

Revision as of 07:07, 2 July 2008

Image:1kcb.png

Template:STRUCTURE 1kcb

Crystal Structure of a NO-forming Nitrite Reductase Mutant: an Analog of a Transition State in Enzymatic Reaction

Template:ABSTRACT PUBMED 12615072

About this Structure

1KCB is a Single protein structure of sequence from Achromobacter cycloclastes. Full crystallographic information is available from OCA.

Reference

Crystal structure of a NO-forming nitrite reductase mutant: an analog of a transition state in enzymatic reaction., Liu SQ, Chang T, Liu MY, LeGall J, Chang WC, Zhang JP, Liang DC, Chang WR, Biochem Biophys Res Commun. 2003 Mar 14;302(3):568-74. PMID:12615072

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