1kfa

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{{STRUCTURE_1kfa| PDB=1kfa | SCENE= }}
{{STRUCTURE_1kfa| PDB=1kfa | SCENE= }}
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'''Crystal structure of Fab fragment complexed with gibberellin A4'''
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===Crystal structure of Fab fragment complexed with gibberellin A4===
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==Overview==
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Gibberellins, a class of plant hormones, consist of more than 120 members. Only a few of them are recognized by a receptor that remains unknown. The haptenic mouse monoclonal antibody, 4-B8(8)/E9, was generated against gibberellin A(4) (GA(4)) to recognize biologically active GA selectivity, and we attempted to confirm the binding properties between the antibody and GA(4). We carried out an X-ray crystallographic analysis of the 4-B8(8)/E9 Fab fragment complexed with GA(4) at a 2.8 A resolution by using the molecular replacement method. The crystal structure of the Fab fragment showed the typical immunoglobulin fold of the beta-barrel structure which is the common motif of all antibodies. A small hapten-combining site was made up of three heavy chain CDR loops. On the other hand, CDRs of the light chain did not interact directly with GA(4). The C/D rings of the GA(4) molecule were in van der Waals contact mainly with the aromatic side chain of Tyr100AH and Phe100BH of CDR-H3. The 3 beta-hydroxyl and 6 beta-carboxyl groups were, respectively, hydrogen-bonded to the main chain of Ala33H and to the Thr53H heavy chain.
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(as it appears on PubMed at http://www.pubmed.gov), where 12054627 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12054627}}
==About this Structure==
==About this Structure==
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[[Category: Yamaguchi, I.]]
[[Category: Yamaguchi, I.]]
[[Category: Immunoglobuiln fold]]
[[Category: Immunoglobuiln fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:40:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:15:21 2008''

Revision as of 07:15, 2 July 2008

Image:1kfa.png

Template:STRUCTURE 1kfa

Crystal structure of Fab fragment complexed with gibberellin A4

Template:ABSTRACT PUBMED 12054627

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structure of the liganded anti-gibberellin A(4) antibody 4-B8(8)/E9 Fab fragment., Murata T, Fushinobu S, Nakajima M, Asami O, Sassa T, Wakagi T, Yamaguchi I, Biochem Biophys Res Commun. 2002 Apr 26;293(1):489-96. PMID:12054627

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