1hr1
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(New page: 200px<br /><applet load="1hr1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hr1" /> '''STRUCTURE OF AN INDOLICIDIN PEPTIDE DERIVATI...)
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Revision as of 14:39, 20 November 2007
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STRUCTURE OF AN INDOLICIDIN PEPTIDE DERIVATIVE WITH P-->A SUBSTITUTION
Overview
Indolicidin, an antimicrobial peptide with a unique amino acid sequence, (ILPWKWPWWPWRR-NH(2)) is found in bovine neutrophils. A derivative of, indolicidin, CP10A, has alanine residues substituted for proline residues, and has improved activity against Gram-positive organisms. Transmission, electron microscopy of Staphylococcus aureus and Staphylococcus, epidermidis treated with CP10A showed mesosome-like structures in the, cytoplasm. The peptide at 2-fold the minimal inhibitory concentration did, not show significant killing of S. aureus ISP67 (a histidine, uridine, and, thymidine auxotroph) but did show an early effect on histidine and uridine, incorporation and, later, an effect on thymidine incorporation. Upon, interaction with liposomes, detergents, and lipoteichoic acid, CP10A was, shown by circular dichroism spectroscopy to undergo a change in secondary, structure. Fluorescence spectroscopy indicated that the tryptophan, residues were located at the hydrophobic/hydrophilic interface of, liposomes and detergent micelles and were inaccessible to the aqueous, quencher KI. The three-dimensional structure of CP10A in the lipid mimetic, dodecylphosphocholine was determined using two-dimensional NMR methods and, was characterized as a short, amphipathic helical structure, whereas, indolicidin was previously shown to have an extended structure. These, studies have introduced a cationic peptide with a unique structure and an, ability to interact with membranes and to affect intracellular synthesis, of proteins, RNA, and DNA.
About this Structure
1HR1 is a Single protein structure of sequence from [1] with NH2 as ligand. Full crystallographic information is available from OCA.
Reference
Structure and mechanism of action of an indolicidin peptide derivative with improved activity against gram-positive bacteria., Friedrich CL, Rozek A, Patrzykat A, Hancock RE, J Biol Chem. 2001 Jun 29;276(26):24015-22. Epub 2001 Apr 9. PMID:11294848
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