1kma

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{{STRUCTURE_1kma| PDB=1kma | SCENE= }}
{{STRUCTURE_1kma| PDB=1kma | SCENE= }}
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'''NMR Structure of the Domain-I of the Kazal-type Thrombin Inhibitor Dipetalin'''
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===NMR Structure of the Domain-I of the Kazal-type Thrombin Inhibitor Dipetalin===
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==Overview==
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The interaction of domains of the Kazal-type inhibitor protein dipetalin with the serine proteinases thrombin and trypsin is studied. The functional studies of the recombinantly expressed domains (Dip-I+II, Dip-I and Dip-II) allow the dissection of the thrombin inhibitory properties and the identification of Dip-I as a key contributor to thrombin/dipetalin complex stability and its inhibitory potency. Furthermore, Dip-I, but not Dip-II, forms a complex with trypsin resulting in an inhibition of the trypsin activity directed towards protein substrates. The high resolution NMR structure of the Dip-I domain is determined using multi-dimensional heteronuclear NMR spectroscopy. Dip-I exhibits the canonical Kazal-type fold with a central alpha-helix and a short two-stranded antiparallel beta-sheet. Molecular regions essential for inhibitor complex formation with thrombin and trypsin are identified. A comparison with molecular complexes of other Kazal-type thrombin and trypsin inhibitors by molecular modeling shows that the N-terminal segment of Dip-I fulfills the structural prerequisites for inhibitory interactions with either proteinase and explains the capacity of this single Kazal-type domain to interact with different proteinases.
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(as it appears on PubMed at http://www.pubmed.gov), where 12051857 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12051857}}
==About this Structure==
==About this Structure==
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1KMA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Dipetalogaster_maximus Dipetalogaster maximus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KMA OCA].
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1KMA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Dipetalogaster_maximus Dipetalogaster maximus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KMA OCA].
==Reference==
==Reference==
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[[Category: Disulphide-rich small alpha+beta fold]]
[[Category: Disulphide-rich small alpha+beta fold]]
[[Category: Kazal-type]]
[[Category: Kazal-type]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:54:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:32:43 2008''

Revision as of 07:32, 2 July 2008

Image:1kma.png

Template:STRUCTURE 1kma

NMR Structure of the Domain-I of the Kazal-type Thrombin Inhibitor Dipetalin

Template:ABSTRACT PUBMED 12051857

About this Structure

1KMA is a Single protein structure of sequence from Dipetalogaster maximus. Full experimental information is available from OCA.

Reference

Interaction of Kazal-type inhibitor domains with serine proteinases: biochemical and structural studies., Schlott B, Wohnert J, Icke C, Hartmann M, Ramachandran R, Guhrs KH, Glusa E, Flemming J, Gorlach M, Grosse F, Ohlenschlager O, J Mol Biol. 2002 Apr 26;318(2):533-46. PMID:12051857

Page seeded by OCA on Wed Jul 2 10:32:43 2008

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