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1kmr

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[[Image:1kmr.gif|left|200px]]
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{{STRUCTURE_1kmr| PDB=1kmr | SCENE= }}
{{STRUCTURE_1kmr| PDB=1kmr | SCENE= }}
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'''Solution NMR Structure of Surfactant Protein B (11-25) (SP-B11-25)'''
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===Solution NMR Structure of Surfactant Protein B (11-25) (SP-B11-25)===
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==Overview==
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Surfactant protein B (SP-B) is a 79-residue essential component of lung surfactant, the film of lipid and protein lining the alveoli, and is the subject of great interest for its role in lung surfactant replacement therapies. Here we report circular dichroism results and the solution NMR structure of SP-B(11-25) (CRALIKRIQAMIPKG) dissolved in CD(3)OH at 5 degrees C. This is the first report of NMR data related to the protein SP-B, whose structure promises to help elucidate the mechanism of its function. Sequence-specific resonance assignments were made for all observable (1)H NMR signals on the basis of standard 2D NMR methods. Structures were determined by the simulated annealing method using restraints derived from 2D NOESY data. The calculations yielded 17 energy-minimized structures, three of which were subjected to 0.95 ns of restrained dynamics to assess the relevance of the static structures to more realistic dynamic behavior. Our CD and NMR data confirm that this segment is an amphiphilic alpha helix from approximately residue L14 through M21. The backbone heavy-atom RMSD for residues L14 through M21 is 0.09 +/- 0.12 A, and the backbone heavy-atom RMSD for the whole peptide is 0.96 +/- 2.45 A, the difference reflecting fraying at the termini. Aside from the disordered termini, the minimized structures represent dynamic structures well. Structural similarity to the homologous regions of related saposin-like proteins and the importance of the distribution of polar residues about the helix axis are discussed.
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The line below this paragraph, {{ABSTRACT_PUBMED_12135384}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12135384 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12135384}}
==About this Structure==
==About this Structure==
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1KMR is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KMR OCA].
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1KMR is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KMR OCA].
==Reference==
==Reference==
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[[Category: Lee, K Y.C.]]
[[Category: Lee, K Y.C.]]
[[Category: Helix]]
[[Category: Helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:55:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:34:06 2008''

Revision as of 07:34, 2 July 2008

Image:1kmr.png

Template:STRUCTURE 1kmr

Solution NMR Structure of Surfactant Protein B (11-25) (SP-B11-25)

Template:ABSTRACT PUBMED 12135384

About this Structure

1KMR is a Single protein structure. Full experimental information is available from OCA.

Reference

NMR structure of lung surfactant peptide SP-B(11-25)., Kurutz JW, Lee KY, Biochemistry. 2002 Jul 30;41(30):9627-36. PMID:12135384

Page seeded by OCA on Wed Jul 2 10:34:06 2008

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