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| - | [[Image:1kn1.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1kn1| PDB=1kn1 | SCENE= }} | | {{STRUCTURE_1kn1| PDB=1kn1 | SCENE= }} |
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| - | '''Crystal structure of allophycocyanin'''
| + | ===Crystal structure of allophycocyanin=== |
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| - | ==Overview==
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| - | The crystal structure of allophycocyanin from red algae Porphyra yezoensis (APC-PY) at 2.2-A resolution has been determined by the molecular replacement method. The crystal belongs to space group R32 with cell parameters a = b = 105.3 A, c = 189.4 A, alpha = beta = 90 degrees, gamma = 120 degrees. After several cycles of refinement using program X-PLOR and model building based on the electron density map, the crystallographic R-factor converged to 19.3% (R-free factor is 26.9%) in the range of 10.0 to 2.2 A. The r.m.s. deviations of bond length and angles are 0.015 A and 2.9 degrees, respectively. In the crystal, two APC-PY trimers associate face to face into a hexamer. The assembly of two trimers within the hexamer is similar to that of C-phycocyanin (C-PC) and R-phycoerythrin (R-PE) hexamers, but the assembly tightness of the two trimers to the hexamer is not so high as that in C-PC and R-PE hexamers. The chromophore-protein interactions and possible pathway of energy transfer were discussed. Phycocyanobilin 1alpha84 of APC-PY forms 5 hydrogen bonds with 3 residues in subunit 2beta of another monomer. In R-PE and C-PC, chromophore 1alpha84 only forms 1 hydrogen bond with 2beta77 residue in subunit 2beta. This result may support and explain great spectrum difference exists between APC trimer and monomer.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_10358042}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10358042 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_10358042}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Zhang, J P.]] | | [[Category: Zhang, J P.]] |
| | [[Category: Helix-turn-helix]] | | [[Category: Helix-turn-helix]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:56:03 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:34:46 2008'' |
Revision as of 07:34, 2 July 2008
Template:STRUCTURE 1kn1
Crystal structure of allophycocyanin
Template:ABSTRACT PUBMED 10358042
About this Structure
1KN1 is a Protein complex structure of sequences from Porphyra yezoensis. Full crystallographic information is available from OCA.
Reference
Crystal structure of allophycocyanin from red algae Porphyra yezoensis at 2.2-A resolution., Liu JY, Jiang T, Zhang JP, Liang DC, J Biol Chem. 1999 Jun 11;274(24):16945-52. PMID:10358042
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