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1ko7

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{{STRUCTURE_1ko7| PDB=1ko7 | SCENE= }}
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'''X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution'''
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===X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution===
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==Overview==
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The histidine containing phospho carrier protein (HPr) kinase/phosphatase is involved in carbon catabolite repression, mainly in Gram-positive bacteria. It is a bifunctional enzyme that phosphorylates Ser-46-HPr in an ATP-dependent reaction and dephosphorylates P-Ser-46-HPr. X-ray analysis of the full-length crystalline enzyme from Staphylococcus xylosus at a resolution of 1.95 A shows the enzyme to consist of two clearly separated domains that are assembled in a hexameric structure resembling a three-bladed propeller. The N-terminal domain has a betaalphabeta fold similar to a segment from enzyme I of the sugar phosphotransferase system and to the uridyl-binding portion of MurF; it is structurally organized in three dimeric modules exposed to form the propeller blades. Two unexpected phosphate ions associated with highly conserved residues were found in the N-terminal dimeric interface. The C-terminal kinase domain is similar to that of the Lactobacillus casei enzyme and is assembled in six copies to form the compact central hub of the propeller. Beyond previously reported similarity with adenylate kinase, we suggest evolutionary relationship with phosphoenolpyruvate carboxykinase. In addition to a phosphate ion in the phosphate-binding loop of the kinase domain, we have identified a second phosphate-binding site that, by comparison with adenylate kinases, we believe accommodates a product/substrate phosphate, normally covalently linked to Ser-46 of HPr. Thus, we propose that our structure represents a product/substrate mimic of the kinase/phosphatase reaction.
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(as it appears on PubMed at http://www.pubmed.gov), where 11904409 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11904409}}
==About this Structure==
==About this Structure==
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[[Category: Protein phosphatase]]
[[Category: Protein phosphatase]]
[[Category: Substrate]]
[[Category: Substrate]]
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Revision as of 07:38, 2 July 2008

Image:1ko7.png

Template:STRUCTURE 1ko7

X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution

Template:ABSTRACT PUBMED 11904409

About this Structure

1KO7 is a Single protein structure of sequence from Staphylococcus xylosus. Full crystallographic information is available from OCA.

Reference

Structure of the full-length HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution: Mimicking the product/substrate of the phospho transfer reactions., Marquez JA, Hasenbein S, Koch B, Fieulaine S, Nessler S, Russell RB, Hengstenberg W, Scheffzek K, Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3458-63. PMID:11904409

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