1ks4

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{{STRUCTURE_1ks4| PDB=1ks4 | SCENE= }}
{{STRUCTURE_1ks4| PDB=1ks4 | SCENE= }}
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'''The structure of Aspergillus niger endoglucanase-palladium complex'''
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===The structure of Aspergillus niger endoglucanase-palladium complex===
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==Overview==
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The fungus Aspergillus niger is a main source of industrial cellulase. beta-1,4-Endoglucanase is the major component of cellulase from A. niger. In spite of widespread applications, little is known about the structure of this enzyme. Here, the structure of beta-1,4-endoglucanase from A. niger (EglA) was determined at 2.1 A resolution. Although there is a low sequence identity between EglA and CelB2, another member of family 12, the three-dimensional structures of their core regions are quite similar. The structural differences are mostly found in the loop regions, where CelB2 has an extra beta-sheet (beta-sheet C) at the non-reducing end of the binding cleft of the native enzyme. Incubation of EglA with PdCl(2) irreversibly inhibits the EglA activity. Structural studies of the enzyme-palladium complex show that three Pd(2+) ions bind to each EglA molecule. One of the Pd(2+) ions forms a coordinate covalent bond with Met118 S(delta) and the nucleophilic Glu116 O(epsilon1) at the active site of the enzyme. The other two Pd(2+) ions bind on the surface of the protein. Binding of Pd(2+) ions to EglA does not change the general conformation of the backbone of the protein significantly. Based on this structural study, one can conclude that the palladium ion directly binds to and blocks the active site of EglA and thus inactivates the enzyme.
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(as it appears on PubMed at http://www.pubmed.gov), where 11914491 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11914491}}
==About this Structure==
==About this Structure==
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[[Category: Endoglucanase]]
[[Category: Endoglucanase]]
[[Category: Family 9]]
[[Category: Family 9]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:52:31 2008''

Revision as of 07:52, 2 July 2008

Image:1ks4.png

Template:STRUCTURE 1ks4

The structure of Aspergillus niger endoglucanase-palladium complex

Template:ABSTRACT PUBMED 11914491

About this Structure

1KS4 is a Single protein structure of sequence from Aspergillus niger. Full crystallographic information is available from OCA.

Reference

Determination of the structure of an endoglucanase from Aspergillus niger and its mode of inhibition by palladium chloride., Khademi S, Zhang D, Swanson SM, Wartenberg A, Witte K, Meyer EF, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):660-7. Epub 2002, Mar 22. PMID:11914491

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