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1ktw

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{{STRUCTURE_1ktw| PDB=1ktw | SCENE= }}
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'''IOTA-CARRAGEENASE COMPLEXED TO IOTA-CARRAGEENAN FRAGMENTS'''
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===IOTA-CARRAGEENASE COMPLEXED TO IOTA-CARRAGEENAN FRAGMENTS===
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==Overview==
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iota-Carrageenans are sulfated 1,3-alpha-1,4-beta-galactans from the cell walls of red algae, which auto-associate into crystalline fibers made of aggregates of double-stranded helices. iota-Carrageenases, which constitute family 82 of glycoside hydrolases, fold into a right-handed beta-helix. Here, the structure of Alteromonas fortis iota-carrageenase bound to iota-carrageenan fragments was solved at 2.0A resolution (PDB 1KTW). The enzyme holds a iota-carrageenan tetrasaccharide (subsites +1 to +4) and a disaccharide (subsites -3, -4), thus providing the first direct determination of a 3D structure of iota-carrageenan. Electrostatic interactions between basic protein residues and the sulfate substituents of the polysaccharide chain dominate iota-carrageenan recognition. Glu245 and Asp247 are the proton donor and the base catalyst, respectively. C-terminal domain A, which was highly flexible in the native enzyme structure, adopts a alpha/beta-fold, also found in DNA/RNA-binding domains. In the substrate-enzyme complex, this polyanion-binding module shifts toward the beta-helix groove, forming a tunnel. Thus, from an open conformation which allows for the initial endo-attack of iota-carrageenan chains, the enzyme switches to a closed-tunnel form, consistent with its highly processive character, as seen from the electron-microscopy analysis of the degradation of iota-carrageenan fibers.
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==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Iota-carrageenan double helix degradation]]
[[Category: Iota-carrageenan double helix degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:59:46 2008''

Revision as of 07:59, 2 July 2008

Image:1ktw.png

Template:STRUCTURE 1ktw

IOTA-CARRAGEENASE COMPLEXED TO IOTA-CARRAGEENAN FRAGMENTS

Template:ABSTRACT PUBMED 14623184

About this Structure

1KTW is a Single protein structure of sequence from Alteromonas sp. atcc 43554. Full crystallographic information is available from OCA.

Reference

The structural bases of the processive degradation of iota-carrageenan, a main cell wall polysaccharide of red algae., Michel G, Helbert W, Kahn R, Dideberg O, Kloareg B, J Mol Biol. 2003 Nov 28;334(3):421-33. PMID:14623184

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