1ku0

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{{STRUCTURE_1ku0| PDB=1ku0 | SCENE= }}
{{STRUCTURE_1ku0| PDB=1ku0 | SCENE= }}
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'''Structure of the Bacillus stearothermophilus L1 lipase'''
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===Structure of the Bacillus stearothermophilus L1 lipase===
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==Overview==
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The bacterial thermoalkalophilic lipases optimally hydrolyze saturated fatty acids at elevated temperatures. They also have significant sequence homology with staphylococcal lipases, and both the thermoalkalophilic and staphylococcal lipases are grouped as the lipase family I.5. We report here the first crystal structure of the lipase family I.5, the structure of a thermoalkalophilic lipase from Bacillus stearothermophilus L1 (L1 lipase) determined at 2.0-A resolution. The structure is in a closed conformation, and the active site is buried under a long lid helix. Unexpectedly, the structure exhibits a zinc-binding site in an extra domain that accounts for the larger molecular size of the family I.5 enzymes in comparison to other microbial lipases. The zinc-coordinated extra domain makes tight interactions with the loop extended from the C terminus of the lid helix, suggesting that the activation of the family I.5 lipases may be regulated by the strength of the interactions. The unusually long lid helix makes strong hydrophobic interactions with its neighbors. The structural information together with previous biochemical observations indicate that the temperature-mediated lid opening is triggered by the thermal dissociation of the hydrophobic interactions.
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The line below this paragraph, {{ABSTRACT_PUBMED_11859083}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11859083 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11859083}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Lipase]]
[[Category: Lipase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:10:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:00:07 2008''

Revision as of 08:00, 2 July 2008

Image:1ku0.png

Template:STRUCTURE 1ku0

Structure of the Bacillus stearothermophilus L1 lipase

Template:ABSTRACT PUBMED 11859083

About this Structure

1KU0 is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

Reference

Novel zinc-binding center and a temperature switch in the Bacillus stearothermophilus L1 lipase., Jeong ST, Kim HK, Kim SJ, Chi SW, Pan JG, Oh TK, Ryu SE, J Biol Chem. 2002 May 10;277(19):17041-7. Epub 2002 Feb 21. PMID:11859083

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