1kuq

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[[Image:1kuq.gif|left|200px]]
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{{STRUCTURE_1kuq| PDB=1kuq | SCENE= }}
{{STRUCTURE_1kuq| PDB=1kuq | SCENE= }}
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'''CRYSTAL STRUCTURE OF T3C MUTANT S15 RIBOSOMAL PROTEIN IN COMPLEX WITH 16S RRNA'''
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===CRYSTAL STRUCTURE OF T3C MUTANT S15 RIBOSOMAL PROTEIN IN COMPLEX WITH 16S RRNA===
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==Overview==
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The position and conformation of the N-terminal helix of free ribosomal protein S15 was earlier found to be modified under various conditions. This variability was supposed to provide the recognition by the protein of its specific site on 16S rRNA. To test this hypothesis, we substituted some amino acid residues in this helix and assessed effects of these substitutions on the affinity of the protein for 16S rRNA. The crystal structure of the complex of one of these mutants (Thr3Cys S15) with the 16S rRNA fragment was determined, and a computer model of the complex containing another mutant (Gln8Met S15) was designed. The available and new information was analyzed in detail, and the N-terminal helix was concluded to play no significant role in the specific binding of the S15 protein to its target on 16S rRNA.
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The line below this paragraph, {{ABSTRACT_PUBMED_15627386}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15627386 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15627386}}
==About this Structure==
==About this Structure==
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[[Category: Tishchenko, S.]]
[[Category: Tishchenko, S.]]
[[Category: Rrna-protein complex]]
[[Category: Rrna-protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:11:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:02:53 2008''

Revision as of 08:02, 2 July 2008

Image:1kuq.png

Template:STRUCTURE 1kuq

CRYSTAL STRUCTURE OF T3C MUTANT S15 RIBOSOMAL PROTEIN IN COMPLEX WITH 16S RRNA

Template:ABSTRACT PUBMED 15627386

About this Structure

1KUQ is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Role of N-terminal helix in interaction of ribosomal protein S15 with 16S rRNA., Revtovich SV, Nikulin AD, Nikonov SV, Biochemistry (Mosc). 2004 Dec;69(12):1319-23. PMID:15627386

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