1kvi

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[[Image:1kvi.gif|left|200px]]
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{{STRUCTURE_1kvi| PDB=1kvi | SCENE= }}
{{STRUCTURE_1kvi| PDB=1kvi | SCENE= }}
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'''Solution Structure of the Reduced Form of the First Heavy Metal Binding Motif of the Menkes Protein'''
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===Solution Structure of the Reduced Form of the First Heavy Metal Binding Motif of the Menkes Protein===
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==Overview==
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The coding sequence for the first N-terminal copper binding motif of the human Menkes disease protein (MNK1; residues 2-79) was synthesized, cloned, and expressed in bacteria for biochemical and structural studies. MNK1 adopts the betaalphabetabetaalphabeta fold common to all the metal binding sequences (MBS) found in other metal transport systems (e.g., the yeast copper chaperone for superoxide dismutase CCS, the yeast copper chaperone ATX1 bound to Hg(II), and most recently Cu(I), the bacterial copper binding protein, CopZ, and the bacterial Hg(II) binding protein MerP), although substantial differences were found in the metal binding loop. Similar to ATX1, MNK1 binds Cu(I) in a distorted linear bicoordinate geometry. As with MerP, MNK1 has a high affinity for both Hg(II) and Cu(I), although it displays a marked preference for Cu(I). In addition, we found that F71 is a key residue in the compact folding of MNK1, and its mutation to alanine results in an unfolded structure. The homologous residue in MerP has also been mutated with similar results. Finally, to understand the relationship between protein folding and metal affinity and specificity, we expressed a chimeric MBS with the MNK1 protein carrying the binding motif of MerP (CAAC-MNK1); this chimeric protein showed differences in structure and the dynamics of the binding site that may account for metal specificity.
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(as it appears on PubMed at http://www.pubmed.gov), where 16211579 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16211579}}
==About this Structure==
==About this Structure==
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1KVI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KVI OCA].
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1KVI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KVI OCA].
==Reference==
==Reference==
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[[Category: Cu-protein]]
[[Category: Cu-protein]]
[[Category: Menke]]
[[Category: Menke]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:13:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:05:37 2008''

Revision as of 08:05, 2 July 2008

Image:1kvi.png

Template:STRUCTURE 1kvi

Solution Structure of the Reduced Form of the First Heavy Metal Binding Motif of the Menkes Protein

Template:ABSTRACT PUBMED 16211579

About this Structure

1KVI is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Solution structures of the reduced and Cu(I) bound forms of the first metal binding sequence of ATP7A associated with Menkes disease., DeSilva TM, Veglia G, Opella SJ, Proteins. 2005 Dec 1;61(4):1038-49. PMID:16211579

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